红外光谱法测定壳聚糖含量.pdf

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1、第24卷第2期化学研究中国科技核心期刊2013年3月CHEM1CALRESEARCHhxyj@henu．edu．cnDeterminationofchitosancontentbyFouriertransforminfraredspectrometryTANGBing—hua(ZhanjiangLifeSourceHealthProductsCompanyLtd．，Zhanjiang524000，Guangdong，China)Abstract：AFouriertransforminfraredspectrometric(FT—IR)methodwasestablished

2、tode—terminethecontentofchitosanwhilethearearatiooftheabsorptionpeaksofmethenylgroupinpyranosering(1389and2865cm一)wascitedasthequantitativereference．Theadaptabil—itvandusabilitvofas—establishedmethodwasvalidated．ResultsindicatethataS—establishedFT—IRmethodcanbewelladoptedtodeterminethecont

3、entofchitosaninpharmaceuticalfor—mulations．Keywords：chitosan；infraredspectroscopy；content；determinationCLCnumber：TQ463Documentcode：AArticleID：1008—1011(2013)02一Ol15—03红外光谱法测定壳聚糖含量唐秉华(湛江健力源医疗用品有限公司，广东湛江524000)摘要：采用毗喃环上的次甲基的吸收峰(1389和2865cm一)面积比作为定量参照，建立了一种检测壳聚糖含量的傅立叶变换红外光谱(FT—IR)分析方法，并验证了其适用

4、性．结果表明，所建立的FT—IR分析方法可用于定量测定药物剂型中的壳聚糖含量．关键词：壳聚糖；红外光谱；含量；测定Chitosan．2-amino一2一deoxy—b一1，4-glucanpreparedbyN—deacetylationofchitin，1‘Sabiodegradableandnon-toxicDolysaccharidewithlowimmunogenicity[．Asthemostimportantderivateofchitin，chitosanpossessesmanvimportantcharacteristicssuchasdefinedche

5、micalstructure，specialphysicalandbiologicalfunction，biodegradability，andbiocompatibility，anditcanbeeasilyprocessedintovariousproductsinclu—dingflakes，finepowders，beads，membranes，sponges，cottons，fibers，andge[s[一引．Therefore，inthe21stcenturv，chitosanfacesnewopportunitiestocontributetofunction

6、almaterialsandenvironmentallyfriendlvmateria1stherebymeetingthediverseneedsoftoday’Ssociety．Inthissense，itisimperativetodevelopnovelefficientmethodsforthedeterminationofchitosan．Common1vusedmethodsforthedeterminationofchitosancontentincludechromatography，spectro—photometry，nuclearmagneticr

7、esonancespectroscopy，colorimetricassayand{luorimetrictechnique，oracombinationofthesemethods~6-1o3Chromatographyhasahighsensitivityandcanbeadoptedtoprovide．accurateandreliableresults，butitistediousandtime—consuming．Spectrophotometry，asatraditionalmethod，hasbeen