拟南芥miRNA172a-1b-2c对多种胁迫响应的研究.pdf

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1、目录摘要……ABS下RAC下................................................................................................................……2第一章弓!言............……，.......................................................................................……31nlicroRNA......

2、...................................................................................................……31.1microRNA概述.....................................................······································……31.2miCroRNA与非生物肋、迫之间的关系........................

3、...............................……31.3microRNA调控植物的生长发育....................................................……，....……42而R172自勺研究状祝及意义.............................................................................……53前期研究.....................................……，

4、...............................................................……7第二章材料一与方法..........................································，·····································……9l材料...............................................................................

5、...................................……91.1拟南芥.......................................................................................................……91.2菌株和载体.，.................................................··.·········································……9

6、1.3试剂和仪器......................................................................··.······················……92方法二，.............................................................................................................……102.IR丁一pCR研究目标基因的表达模式............

7、.............................................……102.2植物表达载体的构建和拟南芥的转化.................................................……n2.3拟南芥的胁迫处理实验..........................................................................……142.4pEG处理后的电导率的测定.............................

8、.....................................……15第三章结果........................................................................................................……16l;11iRNA!72a一1/b一2/c)字列的比对与聚类分析.......................