甜菜品种(系)的ISSR标记数字指纹图谱构建及聚类分析.pdf

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1、第28卷增刊22802012年10月农业工程学报TransactionsoftheChineseSocietyofA伊iculturalEngineeringv01．28Supp．2Oct．2012ConstructionofdigitalfingerprintingandclusteranalysisusingISSRmarkersforsugarbeetcultiVars(1ines)L-uQiaohon91，ChengDayouⅨ，YangL_n1，LuoChengfei1，KongFanjiangz，WuYumeij(1．＆^D0，矿乃耐＆匆珂cP口材E堙f行PP砌lg，^kr6拥

2、而州m把矿他c而胛DfD』奶凰砌加150090，C像加口；2．NormenstInstimte可Geogrnphy册dA矿0ecolo斟，ChmeseAcⅡdemy09fsciences．Harb衲、3响幢，Ch衲dj3．勋2口，Be“R8JP口rc而讹“f“把D厂'C删S胁而砌150080，China)Abstract：ISSRfingeq埘mingandclusteranalysiswereconducted锄ong4in仃oducedsugarbeet∞．v甜国口r括)cultiVars丹omHoll锄dand6lines(including2annualbeets)厅omChina

3、usingISSRmarke腿SiXstableandhighpolymorphicprimeswereselectedinthisstudy．5lbandswereobtainedusingISSR—PCRwiththesixprimers，arldpolymo巾hicrateofmeb锄dswas86．3％．AUtestvarieties(1ines)canbedistinguishedbyISSRfingerprimingusingthesiXprimes．Digitalfingerp血tcodeswerecons仃uctedby8polymorphicbandsfromISSRpr

4、imerL1aIldUBC846，whichcoulddiscmlinate10cultivars(1ines)completely．TheresuhindicatedthatISSRfingerprintingcouldidenti匆dif断entsugarbeetvarictiesemciemly．T1legeneticsimila—tymatrix锄ongaUl0sampleswasobtainedbysimilari够analysisusingDicealgorjtllmwitllNTSYS-pcsO腑are，andsimilaritycoemcientsranged仔om0．43

5、to0．83withanavemgeof0．62．ClustertreewasfomedusingUPGMAmethod．Clustera11alysisshowedthat10varieties(1ines)weredivided铆ogroupsand恤eesub盯oups．UPGMAclusteranalysisshowedcleargeneticrelationships锄ongme10sugarbeetcultivars．Theresultindicatedthatmecluster仃eewasinaccordancewichthechamcterofthepopulationsa

6、11dISsRmarkerscouldbeusedtoestimatcgcneticdistancebe俩eenp叩ulaIions．Keywords：clustera11alysis，agricult【lralproducts，qualitycon仃ol，sugarbeet'ISSRmarkers，finge巾rinting，geneticsimilarit)rdoi：10．3969／i．issn．1002—68192012．z2．049CLCnumber：TP391Documentcode：AArticleID：1002～6819(2012)一Supp．2一0280—05LiuQiao

7、hong，ChengDayou，YangLin，eta1．ConshuctionofdigitalfingerprintingandclusterallalysisusingISSRmarkersforsugarbeetcultiVars(1ines)[J]．TraIlsactionsoftheChineseSocie哆ofA鲥culturalEngineering(TransactionsoftlleCSAE)，201