甜高梁组培苗生根培养的优化.pdf

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1、南方农业学报JOuRNAL0FSOUTHERNAGRlCUITuRE2012，43(2)：155—159ISSN2095—119l：CODENNNXAABhttp：／／ww．nfnyxb．comDOI：10．3969／j：issn．2095一l191．2012．02．155Optimizationofmediaforjn订frDrootinductionindifferentgenotypeS0tSWeetS0r2nUmuvIoGEYu—hong，XIEHong—kai，HANJia—yu(AgriculturalCollege，GuangxiUniversity，Nanni

2、ng530005，China)Abstract：【0bjectiVe】111epresentexperimentwasconductedtooptimizetherootregenerationsysteminsweetso唱huminordertodeve】opaneH’ectivegenetictransf0咖ationpmtoc01．【Method】Difkrentbasicmedia(MS，1／2Ms)anddif玷rentconcentmtionsofgmwthhoHnones(IBA，NAAandABT)wereevaluatedandtheiref玷ctsonv

3、ariousfactorssuchasrootqua】ity，motingra￡e，rootjng￡ime，gro讲hofrootedplant】ets，e把．，weredete肋inedtoobtajnanoptimumroo“ngmedium．【Result】Theoptimumrootingmediumforthesweetsor曲umvarietyBT3and【JT3werefoundasl／2MS+IBA(2．0mg，L)and1／2MS+IBA(3．0mg／L)，respectively．TheI℃generatedmotswerethick，andthemote

4、dplantletsshowedvigorousgrowth．Therootingratesvariedintwovarieties．【conclusion】Itisconcludedthatbestqualityofrootscanberegeneratedonshootsusingl／2MSmedium，supplementedwith2．0or3．0mg／LIBAforvarietyBT3and【』3，respectively．Keywords：sweetsorghum；plantletmoting；optimization；plantho珊one；basicmediu

5、mCLCnumber：S514．035．3Doc唧entcode：AArticle：2095—1191(2012)02—0155—05甜高梁组培苗生根培养的优化葛玉红，谢鸿锴，韩佳宇(广西大学农学院，南宁530005)摘要：【目的】优化能源作物甜高粱组培苗的生根培养体系，为遗传转化奠定基础。【方法】利用不同基本培养基(MS、1／2MS)对组培苗生根的影响进行筛选试验，得到较有利于生根的基本培养基；在最佳培养基中添加不同浓度的植物激素(IBA、NAA、ABT)，综合根的质量、生根率、生根时间、生根苗的长势等筛选最佳生根培养基配方。【结果】筛选出最佳生根培养基组合：北甜三号(BT

6、3)为1／2Ms+2．Omg，LIBA，辽甜三号(LT3)为1／2MS+3．0mg／LIBA，且根粗、苗壮，两品种的生根率存在差异。此外，未加激素的Ms和1／2Ms培养基均无组培苗生根。【结论】在1／2Ms上添加2．om玑IBA或3．0m∥LIBA分别为北甜三号、辽甜三号组培苗生根的最佳途径。关键词：甜高粱；组培苗生根；优化；植物激素；基本培养基Sweetsorghumiswjdelyconsjderedasoneofthemostoutstandingenergycrop．Itsphotosyntheticefficiencyis2or3“meshigherthanthat

7、ofwheatandmanyothercrops．ItcanbeusedasagI．ainaswellasasugar—energycrop．Butsofar，thereareverylesssweetsorghumVarietieswhichpossesssuperiorqualitytraits．Maqbooleta1．(2001)advocatedtheimprovementofsorghumusingbiotechn010百calt001sandtechniques．Transgenictech