广西番茄烟粉虱传双生病毒的分布及遗传多样性分析.pdf

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1、南方农业学报JournalofSouthernAgriculture2017，48(1)：72-81ISSN2095—1191；CODENNNXAABhttp：／／www．nfnyxb．comDOI：10．3969／j：issn．2095—1191．2017．01．72Distributionandgeneticdiversityoftomato-infectingbegomovirusesinGuangxi，ChinaLIZhan-biao，XUPeng—chao，QINBi—xia，SUQin，XIEHui-ting，CUILi—xian．CAIJian—he8(InstituteofPl

2、antProtection，GuangxiAcademyofAgriculturalSciences／GuangxiKeyLaboratoryofBiologyforCropDiseasesandInsectPests，Nanning530007，China)Abstract：【ObjectiveJrhepresentstudyinvestigatedvirusspecies，distributions，andmixed-infectionsoftomato-infectingbegomovirusesinGuangxi，inordertoprovidetheoreticalsupportfo

3、rtomatobreedingandrationaldistributionoftomatoanti—virusvariety．【Method】Since2011，thestudyhaveinvestigatedandidentifiedbegomoviruseswhichcausedtomatoyellowleafcurldisease(TYLCD)inGuangxi，andcollected189sampleswithtypicalsymptomsofbegomovirusesinfection．TotalDNAwereextractedandalIthesamplesweredetect

4、edusingdegenerateprimersofbegomoviruses．PCRproductsofpositivesampleswereselected．purifiedandsequenced撕erlinkingtoclonevector．ThesequenceswerecomparedtothoseinGenBank．Virusisolatesfromdifferentplaceswereselected．full—lengthamplificationanddetectionoftheseisolateswereconducted．Theresearchersalsoestabl

5、ishedphylogenetictreestostudytheirevolutionaryrelationshipsandgeneticdiversity．【Result】AccordingtoPCRdetection，therewere139positivesamplesoutof189tomatosamples，positiveratebeing73．54％．Typicaltomatosampleswereselectedandsequenced，fourkindsofbegomovirusesthatcausedTYLCDinGuangxiwerefound：Tomatoleafcur

6、lChinavirus，PapayaleafcurlChinavirus．TomatoyellowleafcurlChinavirusandAgeratumyellowveinChinavirus．Complicatedmixedinfectionsweredetectedinthesamplesandat1eastseventypesofmixedinfectionswerefound．Forty—sevenfullDNA—Asequencesofvirusisolateswereobtained．Phylogenetictreeanalysisindicatedthatvirusisola

7、tesfromthesameregionbelongedtothesamesub-clade，andshowedrichgeneticevolutionaryrelationship．【Conclusion】Afterinvestigationandidentification，fourkindsoftomato—infectingbegomovirusesarefoundexistinginGu