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1、正常妊娠和子痫前期胎盘Syncytin表达差异探讨作者：赵杨,韩凤珍，刘益芬,陈敦金单位：1•广东省人民医院妇产科〃广东省医学科学院，广东广州510080；2.广州医学院附属第三医院妇产科，广东广州510150【摘要】【目的】探讨syncytin及其受体ASCT2在子痫前期(PE)和正常妊娠胎盘组织中的表达改变。【方法】纳入研究病人共37例，分为2组，PE组21例，正常对照组16例，采用定量RT-PCR方法检测syncytin及ASCT2mRNA的转录水平，Westernblot法测定syncytin蛋白表达强度，比较两组较检测结果。【结果】两组均检测到syncytin
2、mRNA、syncytin蛋白及ASCT2mRNA,PE组胎盘syncytinmRNA水平(1.5±1・4)和蛋白表达强度(55.7±26.1)明显低于正常对照组(6.2±3.0,92.2±36.4,P<0.01),ASCT2mRNA水平无明显差异(P>0.05)。【结论】胎盘组织Syncytin表达下调与PE发病密切相关，其受体ASCT2与PE发生无相关性。【关键词】了痫前期；syncytin；ASCT2DifferentialExpressionofSyncytininPlacentabetweenNorma1
3、PregnancyandPreeclampsiaZHAOYang1,HANFeng-zhenl,LIUYi-fen2,CHENDun-jin2(1.DepartmentofObstetricsandGynechology,GuangdongProvincia1Peop1e′sHospitd1,Guangzhou510080,China；2.DepartmentofObstetriesandGynecho1ogy,TheThirdAffi1iatedHospita1,GuangzhouMedica1College,Guangzhou510150,China)A
4、bstract：【Objective】Toexp1oretheexpressionchangesofsyncytinanditsreceptorASCT2inplacentabetweenpreec1ampsia(PE)andnormalpregnancy.【Methods】Thirty-sevenpregnantwomenwereenrolledtotheretrospectivestudyandweredividedintotwogroups・PEgroupwas21placentalspecimensfromPE；normalcontro1gr0upwas16pl
5、acentalspecimensfromnorma1pregnancy・RealtimePCRwasadoptedtodetectthetranscriptionlevelofsyncytinandASCT2mRNAandWesternblotwasusedtodetecttheproteinexpressionofsyncytin.Detectionresu1tsofthetwogroupswerecompared・【Resu1ts】SyncytinmRNA,syncytinProtein,andASCT2mRNAweredetectedinbothgroups・Sy
6、ncyt1nmRNAlevel(1.5±1.4)andproteinexpression(55.7±26.1)ofplacentainPEgroupweresignificantlylowerthanthoseinnormalcontrolgroup(6.2&plusinn;3.0,92.2±36.4,P<0.01),butASCT2mRNAlevelsshowednosignificantdifferenceinthetwogroups(P>0.05).【Conclusion】Down-regu1atedexpressiono
7、fsyncytininp1acentaiscloselyrelatedtotheoccurrenceofPE,butthereceptorASCT2mRNAhasnoassocidtionwithPE.Keywords：preec1ampsia；syncytin；ASCT2[JSUNYat-senUniv(MedSci),2009,30(2)：211-214]子痫前期(preec1ampsia,PE)是产科常见妊娠合并症，其发病原因尚无定论，日前认为胎盘细胞滋养细胞(cytotrophob1ast,CT)生长分化异常是重要原因之一。Sha
