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1、J.Vet.Med.B42,147-154(1995)01995BlackwellWissenschafts-Verlag,BerlinISSN0931-1793DepartmentofVeterinaryMicrobiology,IwateUniversity,Morioka,JapanIsolationandCharacterizationofSialodacryoadenitisVirus(Coronavirus)fromRatsbyEstablishedCellLineLBCN.HIRA
2、NO,K.ONO,R.NOMURAandT.TAWARAAddressofauthors:DepartmentofVeterinaryMicrobiology,IwateUniversity,Morioka020,JapanWith2jiguresand3tables(ReceivedforpublicationAugust18,1994)SummaryTheoutbreakofsialoadenitisoccurredinalaboratoryratcolonyandthecausativea
3、gentwasisolatedfromtheaffectedsalivaryglandsofdiseasedratsusingtheestablishedcelllineLBC.Theisolatereadilymultiplied,producingclearcytopathiceffectswithsyncytiumformation,anditwasidentifiedvirologicallyandserologicallyasratsialodacryoadenitisvirus.In
4、attemptstoisolatethevirusbyprimaryratkidney(PRK)cellsandsucklingmiceaswellasLBCcells,theLBCcellsshowedhighersusceptibilityforthevirusgrowthascomparedwithPRKcellsorthebrainofsucklingmice.Theisolationrateofviruswas100%(5/5)inLBC,40?O(2/5)inPRKcellsand6
5、0%(3/5)insucklingmice.AfterfourpassagesintheLBCcells,thevirusdidnotproducediseaseinadultrats,whilethemousebrain-passagedvirusdid.IntroductionSialodacryoadenitisvirus(SDAV)(BHATI'etal.,1972)isamemberofthecor-onavirusgroup(SIDDELetal.,1983),andisdistri
6、butedworldwideinratcolonies.TheoutbreakofSDAVinfectionhasbeenreportedinmanyratcolonies(UTSUMIetal.,1978,1980;KOJIMAetal.,1980;MARUandSATO,1982;YAMAGUCHIetal.,1982).Thevirussharescommonantigenwithratcoronavirus(RCV)(PARKERetal.,1970)andmousehepatitisv
7、irus(MHV)(BHATTetal.,1972;SIDDELetal.,1983).Sero-monitoringiswidelyconductedfordetectionofinfectionsinbreedingandexperi-mentalcoloniesoflaboratoryratsandmice(UTSUMIetal.,1978,1980;FUJIWARAetal.,1980;NAKAGAWAetal.,1984;MACHIIetal.,1988),suggestingthat
8、inapparentinfectionwithSDAV,RCVorMHVmightbeperpetuatinginratcolonies(UTSUMIetal.,1978,1980;KOJIMAetal.,1980;MARUandSATO,1982;YAMAGUCHIetal.,1982).FromaffectedratsSDAVhasbeenisolatedbyinoculationintothebrainofsucklingmice(KOJIMAetal.,1980;MARUandSATO,
