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1、©ELSEViERAnn.Inst.Pasteur/Virol.Paris19871987,138,315-330ETUDECOMPAREEDETROISSOUCHESDUCORONAVIRUSDELAGASTROENTERITETRANSMISSmLE:CONDITIONSDELAREPLICATIONVIRALEETDELASYNTHESEDESANTIGENESSTRUCTURAUXparT.D.Nguyen(*),S.Bernard,E.Bottreau,I.LantieretJ.M.Aynaud(**)INRA,LaboratoiredePathologiePorcin
2、e,37380Nouzilly(France)SUMMARYCOMPARISONOFTHREESTRAINSOFTHETRANSMISSIBLEGASTROENTERITISCORONAVIRUS(TOEV):VIRALREPLICATIONANDSYNTHESISOFSTRUCTURALANTIGENSPurdue-115andD-52strainsofTOEVwerecomparedwiththe188-S0strain,whichwasobtainedbymeansofasurvivorselectionprocessingastricjuiceofadultpig.The
3、188-S0strainwascharacterizedby(a)lowinfectivity,(b)delayedandrestrictedgrowthassociatedwithlowanddelayedRNAsyn•thesis,and(c)ahighcontentofstructuralantigens.Incontrast,Purdue-l15andD-52strainswerecharacterizedby(a)highinfectivity,and(b)anormalpatternofvirusreplicationandRNAandstructuralantige
4、nsynthesis.TunicamycininducedtheinhibitionofsynthesisofElandE2glycoproteins(detectedbytheELISAtestusingmonoclonalandpolyclonalantibodies)aswellasasignificantreductionintheNPprotein.Theinhibitoryeffectoftunicamycinwasinfluencedbythecelltypeandvirusstrain.KEY-WORDS:Coronavirus,Transmissiblegast
5、roenteritis,Antigen,Tunicamycine;Strains,Replication,ELISA,Infectivity,Pig.ManuscritsoumisIe30aout1986,accepteIe19mars1987.(.)Adresseactuelle:InstitutNationaldeRecherchesVeterinairesNgaTuVong-DongDa,Hanoi(Vietnam).(••)Auteuraquilesdemandesdetiresapartpeuventetreadressees,316r.o.NGUYENETCOLL.I
6、NTRODUCTIONLagastroenteritetransmissible(GET)estuneenteriteneonatalemortelle,hautementcontagieuse,causeeparuncoronavirusspecifiquedel'especepor•cine;IaGETestresponsabledelourdesperteseconomiquesenproductionporcine.LarelationentreIepouvoirpathogeneduvirusetd'eventuelsmar•queursinvitroliesparex
7、empleauxconditionsdesareplication,faitencorel'objetdecontroverse[9,11,14,17].Apresuncertainnombredepassagesenseriesurculturecellulaire,levirusOETperdunegrandepartiedesonpouvoirpathogene.Desresultatsrecentssuggerentqu'Iln'estpaspossiblededisti
