转化酶与甜瓜果实膨大及糖分积累相关研究.pdf

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1、南方农业学报2011，42(4)：384-387JournalofSouthemAgriculture转化酶与甜瓜果实膨大及糖分积累相关研究罗海玲-，一，黄玉辉1，一，张曼-，王日升L2，黄如葵1，何龙飞，(1广西农业科学院蔬菜研究所。南宁530007；2广西作物遗传改良生物技术重点开放实验室，南宁530007；3广西大学农学院，南宁530005)摘要：【目的】探明厚皮甜瓜果实及叶片转化酶与果实膨大及糖分积累的内在机理，为厚皮甜瓜品质遗传改良提供理论依据。【方法】以丰蜜二号和好运52为材料，对甜瓜果实农艺性状、可溶性糖含量、转化酶及叶片转化酶活性进行测定。【结果】两个厚皮甜瓜在授

2、粉后16d内果实纵径长度、横径宽度及果肉厚增长迅速，还原糖含量迅速增加，果肉和叶片酸性转化酶和中性转化酶活性急剧下降，蔗糖含量低；授粉16d后，蔗糖含量迅速增加，果肉酸性转化酶和中性转化酶的活性下降，但叶片中性转化酶活性呈上升趋势。【结论】两个厚皮甜瓜在授粉后16d内，果实迅速膨大，24d后果实膨大速度缓慢，甜瓜蔗糖积累迅速，果肉转化酶活性降到较低水平；相关分析表明，两个厚皮甜瓜品种的还原糖含量与果肉中性转化酶呈显著负相关；在丰蜜二号中，蔗糖积累与叶片中性转化酶呈显著正相关。关键词：甜瓜；果实膨大；蔗糖积累；转化酶；相关分析中图分类号：S652．0l文献标识码：A文章编号：209

3、5—1191(2011)04-0384-04Therelationshipbetweeninvertaseactivityandsucrosel·-l‘』●●■J●●n●J●accumulationdurin2tnedeVel0Dment0ItrUltSlnmelonLUOHai-ling1”，HUANGYu-huil”，ZHANGManl，WANGRi—sheng1”，HUANGRu-kuil，HELong_fei3(1VegetableResearchInstitute，GnangxiAcademyofAgriculturalSciences，Naming530007，Chi

4、na；2GuangxiCropGeneticImprovementandBiotechnologytab，Nanning530007，China；3AgriculturalCollege,GnangxiUniversity，Nanning530005，China)Abstract：【Objective]ThepresentexperimentW88conductedtoelucidatetherelationshipbetweeninvertaseactivityinfruitsandleaves．andflue础accumulationanddevelopmentinmelon

5、topmvidtreferenceforitsqualityimprovement．【Method]Someagronomictraits，solublesugarcontentinfruitsandtheactivityofinvertaseenzymeinleavesandfruitsweredeterminedatdifferentgrowingstagesofmelonvarietiesFengmi2andHaoyun52．【Result]Thefruitoftwomelonvarietiesstartedtodevelopedwithin16dofpollination

6、．Atthisstage，anincreaseinreducingsugarcontentwereoh-served．theactivitiesofacidinvertaseandneutraliflvertaseinfruitsandleavesweredecreasedsharply．AndthesucrOSecontentwasverylowduringthisperiod，however．itincreasedrapidlyafterthis．Theactivitiesofacidinvertaseandneu—tralinvertaseinpulpdeclinedaft

7、er16dofpollination。however，theactivityofneutralinvertaseinleavesshowedrisingtrend．【Conclusion]Thefruitoftwomelonvarietiesdevelopedrapidlywithinthe16dafterpollination，however，itsgrowthratesloweddownat24dafterpollination．Duringthisperiod，thesuc