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时间:2020-03-25
《敌敌畏杀虫剂对桃树叶际微生物群落结构的影响.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、农业环境科学学报2010,29(5):864-869JournalofAgro-EnvironmentScience敌敌畏杀虫剂对桃树叶际微生物群落结构的影响荆梦1,一,宿燕明1,一,谷立坤2,彭霞薇1,李林2,白志辉2(1.北京林业大学,北京100083;2.中国科学院生态环境研究中心,北京100085)摘要:采用平板计数法和磷脂脂肪酸(PLFA)分析方法评价了喷施敌敌畏杀虫剂后对桃树叶际微生物群落的影响。平板计数法分析结果表明,经80%敌敌畏乳油的1000倍液喷雾处理后,可培养微生物数量低于喷水对照。PLFlA分
2、析结果显示,桃树叶际真菌标记物18:k09t磷脂脂肪酸(PLFAs)含量最高,超过总PLFAs含量的60%;喷施杀虫剂后,叶际微生物PLFAs的含量、种类均有所增加,明显有别于喷水对照;并且增加敌敌畏处理次数会增强其对叶际微生物群落影响的显著性。PLFAs主成分分析表明,处理ld后不同样品的叶际微生物群落结构差异最明显,7d后,不同处理样品的叶际微生物群落结构差异变小。关键词:桃树;叶际;敌敌畏;微生物群落;磷脂脂肪酸中图分类号:X172文献标志码:A文章编号:1672—2043(2010)05_0864—06Eff
3、ectsofDichlorovosInsecticideontheMicrobialCommunityinPeachPhyliosphereJINGMen91tSUYan—ming”,GULi—kun2,PENGXia—wei。,LILin2,BAIZhi—hui2(1.BeijingForestryUniversity,Beijing100083,China;2.ResearchCenterforEco—EnvironmentalSciences,ChineseAcademyofSciences,Beijing10
4、0085,China)Abstract:Althoughdichlorovos(DDVP)isoneofthemostwidelyusedorganophosphornsinsecticides,littleisknownregardingtheimpactsofapplyingtheinsecticideonthemicrobialcommunityinplantphyllosphere.Inthepresentstudy,theimpactofDDVPtreatmentonthemi—crobialcommuni
5、tyofpeachphyllospherewasassessedusingtheculture——dependentmenthodofdilution-platecountingandtheculture——inde’_pendenttechniqueofphospholipidfattyacid(PLFA)analysis.Dilution-platecountingassayindicatedthatthenumberofcuhurablemicroor-ganismsinpeachphyllospheretre
6、atedbyDDVPinsecticidew鹊lessthanthatinthewatertreatment.Whereas.PU强analysissuggestedthatDDVPinsecticidetreatmentledtoasignificantincreasedinbethtotalbiomassandspecies.PLFAprofilesalsoindicatedthatthefungalan—saturatedPLFA(18:loJgt)waspredominant(>60%ofthetotalPL
7、FAs)inpeachphyHosphere.PrincipalcomponentanalysisofPLFAsdataindicatedthatDDVPtreatmentdidchangethephyllospheremicrobialcommunitystructuresignificantly;thephyllospheremicrobialeommu—nifiesofDDVPandwatertreatmentswerethemostdifferentafter】daytreatment,andbecamesi
8、milarafter7days.Keywords:Prunuspersica;phyllosphere;dichlorovos;microbialcommunity;phospholipidfattyacid敌敌畏(dichlorovos),化学名称:0,0一二甲基一0一(2,2一二氯乙烯基)磷酸酯(2,2-dichlorovinyldimet
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