应用近红外(NIRS)技术分析小样品油菜籽粒含油量及硫甙含量.pdf

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1、南方农业学报JOURNAL0FSOUTHERNAGRICUIJTUREISSN2095—119l：CODENNNXAABDOI：10．3969／j：issn．2095—1191．2013．1．28应用近红外(NIRS)技术分析小样品油菜籽粒含油量及硫甙含量张美华，刘丽，俎峰，王敬乔+(云南省农业科学院经济作物研究所，昆明650205)摘要：【目的】对193份甘蓝型油菜籽粒进行常量样品(3．0g)及小量样品(0．3g)含油量及硫甙含量近红外光谱分析技术(NIRs)分析和回归模型研究，以期在育种过程中建立实用的油菜小样品含油量及硫甙含量测定数学模型。【方法】利用NIRs对甘

2、蓝型油菜籽粒进行含油量及硫甙含量测定，并进行回归分析。【结果】在众多回归模型中，对于小量样品籽粒含油量及硫甙含量，二次模型均为最优拟合模型，回归方程式分别为：y=40．190+0．892x一0．007x2(R2=o．970)；v=一92．040+o．748x+o．002x：(R2_o．960)(v为常量测量结果，x为小量测量结果)。【结论】获得的二次回归方程式可以很好地将小量样品测定数据转化为常量样品分析结果，为高含油量和低硫甙油菜品种的选育奠定基础。关键词：近红外；甘蓝型油菜；含油量；低硫甙；回归分析中图分类号：s565．4文献标志码：A文章编号：2095一1191(

3、2013)0l一0028—05AnalysisofglucosinolatescontentinsmaUrapeseedsamplesbyusingtheNearInfraredRenectanceSpectroscopy(NIRS)technologyZHANGMei—hua，LIULi，ZUFeng，WANGJing—qiao+(EconomicCmpResearchInstitute，YunnanAcademyofAgricultureSciences，Kunming650205，China)Abstract：【0bjective】Inthisstudy，theo

4、ilcontentandglucosinolatecontentof193．f；rassjcan∈lpusseedsamples(3．0g)andsmaUsamples(013g)wereanalyzedthroughtheNIRStechnologyandtheirregressionmodelswerealsostudiedinordertoestablishmathematicalmodelsfbrthepmcticaldetectionofoilcontentandglucosinolatecontentinsmaUmpeseedsamples．【Method】

5、TheoilcontentandglucosinolatecontentinBrassican母pusseedsweredetenIlinedbyutilizingtheNIRstechnolof拶，andthenthesPSS18．0softwaIewasusedtocaITyouttheregressionanalysis．【Result】withinthemanyre—gressionmodelsfordete瑚iningtheoilcontentandglucosinolatecontentinsmallrapeseedsamples，thequadraticm

6、odelwasthebestfittingmodel，andtheregIessionequationwasrespectiVelyy=40．190+0．892x一0．007x2(R2=O．970)andy=一92．040+0．748x+0．002x2(R2=0．960)(yrepresentstheconstantmeasurementresults，xrepresentssmanfiguresmeasure—mentresults)．【conclusion】Thequadraticregressionequa“oncouldefkctivelytmnsferthes

7、mausampledataresultsintoconstantconversiondataanalysisresults，thereforeabletolaythefoundationforbreedingBrassjca门aPusVarietieswithhighoilcontentand10wglucosinolatecontent．Keywords：NearInfraredRenectanceSpectmscopy(NIRS)；B馏ss记af】apus；oilcontent；glucosinolatescontem；re甜‘ess