基于cDNA-SCoT差异显示的甘蔗应答水分胁迫基因表达谱分析.pdf

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1、南方农业学报JournalofSouthernAgriculture2016，47(12)：1999—2008ISSN2095～1191；CODENNNXAABhttp：／／www．nfnyxb．comDOI：10，39690：issn．2095-1191，2016．12，1999GeneexpressionanalysisofsugarcaneresponsetowaterstressbasedoncDNA-SCoTdifferentialdisplaywuKai—cha01，HUANGCheng-mei2，DE

2、NGZhi-nianl，WEIYuan-wen2，CAOHui-qin92，XULinl，JIANGSheng—li2，WUJian—ruin91，YANGLi—ta03，LIYang-ruil+(1SugarcaneResearchInstitute，GuangxiAcademyofhgriculturalSciences／KeyLaboratoryofSugarcaneBiotechnologyandGeneticImprovement(Guangxi)，MinistryofAgricuIture／Guangx

3、iKeyLaboratoryofSugarcaneGeneticImprovement，Nanning530007，China；2GuangxiCropGeneticImprovementandBiotechnologyLaboratory，Nanning530007，China；3GuangxiUniversity／GuangxiKeyLaboratoryofSubtropicalBioresourceConservationandUtilization，Nanning530004，China)Abstract：

4、【Objective】Thepresentstudywasconductedtostudytheexpressionofdifferentialgenesinsugarcaneresponsetowaterstressanditsmolecularmechanisminordertoprovidescientificbasisfordevelopinggeneticimprovementofsugarcanedroughtresistantintegrity．【Method】SugarcanecultivarGTl

5、1wasusedasmaterialtodesignthreetreatmentsviz．contr01．droughtandrewatering．The+1“leavesofsugarcaneforthreetreatmentswereusedforextractingtotalRNAtoconstructtheirequalquantitymixedsolutions，andthegeneticexpressingprofilesofcuhivarGTl1atelongationstageunderwaters

6、tresswereconstructedbyusingeDNA-SCoTdifferentialdisplay．Thetranscriptderivedfragments(11DFs)werescreenedandisolatedtosequencing，followedbytheBLASThomologysearchinginNCBIdatabasetodeducegenefunctionbasedonhomologicalgenes．【Result】The180TDFswerescreenedbyusing42

7、primersofSCoTmarker，ofwhich100TDFswereofhighersimilaritywiththeaccessedgenesinNCBIdatabase．Accordingtohomologousgenefunction，these100TDFscouldbeclassifiedinto14types，whichincluded1metabolismrelatedgenes，12energymetabolismrelatedgenes，5transportpathwayrelatedge

8、nes，6communicationandsignaltransductionrelatedgenes，3celtcycleandDNAprocessingrelatedgenes，3transcriptregulatedfactorrelatedgenes，17proteinsynthesisrelatedgenes，1proteinprocessingr