p53及VEGF在前列腺癌组织中表达及临床意义.doc

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1、p53及VEGF在前列腺癌组织中表达及临床作者：阎洪涛龚百生董丹丹杨红陈照祥【摘要】目的研究p53和血管内皮生长因子(vascularendothelialgrowthfactor,VEGF)在同一前歹寸腺癌(prostatecancer,Pea)组织中的表达及与Pea临床参数的关系，探讨肿瘤血管形成及调节机制。方法应用免疫组织化学LDP法检测46例Pea组织及20例良性前列腺增生症(benignprostatichyperplasia,BPH)组织中p53蛋白及VEGF的表达。结果Pea组织中p53与VEGF阳性表达率分别为41.30%(19/46)和71.74%(33/46),均明

2、显髙于BPH组(P<；0.05)；p53与VEGF表达呈明显正相关(P<；0.05)；二者在Pea组织中的表达水平与其病理分级和临床分期均呈正相关(P<；0.05)；p53和VEGF阳性表达的肿瘤复发迅速、易转移，p53阴性而VEGF阳性表达较阴性表达者预后差。结论p53和VEGF与Pea组织学分级、恶性程度和预后密切相关，是检测Pea的较好分子标志物；Pea是典型的血管依赖性病变，p53可能通过p53VEGF调节旁路途径促进Pea的肿瘤血管形成，联合检测p53和VEGF的表达可作为判断Pea生物学行为及预后的重要指标。【关键词】前列腺癌p53基因血管内皮生长因子免疫组织化

3、学Theexpressionofp53andVEGFinhumanprostaticcarcinomaandtheirclinicalsignificancesABSTRACT:ObjectiveTostudytheexpressionofp53andvascularendothelialgrowthfactor(VEGF)inhumanprostaticcarcinomatissueandtheirrelationshipwiththeclinicopathologicalparameters,andtounderstandtheroleofp53andVEGFintheprogre

4、ssionoftumors・MethodsTheexpressionofp53andVEGFin46casesofprostaticcarcinomaand20casesofBPHweredeterminedbyimmunohistochemicalLDPmethods・ResuItsThepositiveexpressionratesofp53andVEGFin46casesofPeawere41.30%and71.74%,respectively.Theexpressionlevelsofp53andVEGFinprostaticcarcinomaweremuchhighertha

5、nthoseinBPH(P<；0.05)；thepositivecorrelationwasnotedbetweenp53expressionandVEGFexpression(P<；0・05)；andbothofthemweresignificantlyrelatedtothetumorpathologicgrades(P<；0.05)andtumorclinicalstage(P&It；0.05).Tumorswithhighlevelsofp53thatwereVEGFpositiverecurredearlierandhadagreaterriskofstagepr

6、ogressionthanthosewithlowlevelsofp53andVEGFnegative.ThehighlevelsofVEGFalsoindicatedthepoorestprognosisinp53negativetumorsthanthosewithlowlevelsofVEGF.ConclusionThisstudyindicatesthatp53andVEGFarecloselyrelatedtothehistopathologicalgrading,thetumordifferentiationandprognosisofPea・p53andVEGFarego

7、odmolecularmarkersofPea・Thepathologicalchangesinthetumoraretypicallyvesseldependent,andvascularizationisfavourabletotumorgrowth,infiltrationandrecurrence・Mutemttypep53proteinaccumulationmaybeassociatedwithtumorangiogenesisth