《大鼠颈静脉采血》PPT课件.ppt

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1、JugularBloodCollectioninaConsciousRatCalmtheratdownbeforerestrainingbygentlystrokingtheanimal.Restraintheratbygraspingtheskinoverthenapeoftheneckandshoulderregionwithyourthumbandindexfinger(scruffing).Onceyouhavescruffedtheneck,gentlyturnyourhandoversotheratislyin

2、gcomfortlyinthepalmofyourhand.Donotsqueezetherataroundthethoraxregionbecausethatcouldcausedifficultybreathing.Makesuretherat’sbodyisstraightandnotbentorcurledinyourpalm.Withyourotherhand,useindexfingertolightlyholdheadoutofway.Donotputtomuchpressureonrat’sheadorne

3、ck.Withthumb,holdforelimbsback,whileanothertechnicianshavesthearea.Haveothertechnicianwipedowntheshavedareawithanalcoholswab.Withyourotherhand,useindexfingertolightlyholdhead.Donotputtomuchpressureonratsheadorneck.Withthumb,applyslightpressureonveinproximaltotheco

4、llectionsitesoitwilldistend.Thiswillmakethejugularveinmoreprominentforeasiervisualization.Thebottomhalfofratisstilljustrestinginpalmofhand.Havethetechniciandrawingbloodgetthesyringeandneedleready.Weusea1mlsyringewitha23gaugeneedle.Insertneedlethroughskinrightaroun

5、dthemiddlepointbetweenthesternumandshoulderarea,whereyoushouldseethevein.Advanceslowly.Createaslightvacuumwithinthesyringe,whileadvancingyourneedle.Onceyouhitthevein,asmalldropofbloodwillleakintothehuboftheneedle.Trynottoletyoursyringemovearoundandkeepdrawingbacko

6、ntheplunger.Donotpullbacktoofastbecauseitmightcausetheveintocollapse.Onceyouhaveobtaineddesiredamountofblood,applyaslightamountofpressuretothepuncturesite.