病毒纯化试剂盒说明书.pdf

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1、ContentsIntroduction........................................................................2Beforestarting..................................................................2Kitcomponents..................................................................3Safetyconsiderations........................

2、...............................3Materialsnotsupplied.......................................................3RVPurificationproocol....................................................4Regenerationofcolumn..................................................5Troubleshootingguide.........................

3、...........................6Limiteduseandwarranty.................................................8Page1TMBiomigaViraTrapRetrovirusPurificationMiniprepKitIntroductionTMTheViraTrapretrovirusminipurificationkitisdesignedforfastandefficientpurificationofrecombinantretrovirusesfromretrovirus-transfect

4、edcellculture10supernatant.Upto3x10viralparticlescanbepurifiedfromcellculturemediaof1to2T75flasks.TraditionallytherecombinantretrovirusispurifiedbyultracentrifugationusingCsCltoseparatethevirusparticlesfromcellularproteinsandmediacomponents.TheCsClultracentrifugationprocedureistimeconsumingandlimi

5、tedtotheamountofcelllysatetobeprocessed.Eachcolumncanberegeneratedforpurifyingthesameretrovirus.Foroptimizedviralbindingandrecovery,eachcolumncanberegeneratedonlyonce.BeforeStartingFamiliarwitheachstepbyreadingthismenuandprepareallmaterialsfortheprocedure.Page2TMBiomigaViraTrapRetrovirusPurificati

6、onMiniprepKitKitContentsCatalog#V1172-00V1172-01V1172-02NotesPreps21020RVMiniColumns1510Storeat4°CPress-OnCap2510StoreatRT*Centrifugalfilter2510StoreatRT15mLCollectionTube21020StoreatRT10xWashBuffer10mL30mL60mLStoreatRT2xElutionBuffer10mL30mL60mLStoreatRTRegenerationBuffer10mL30mL60mLStoreatRT*Cen

7、trifugalfilterscanbepurchasedfromBiomigaseparately.StabilityTheGuaranteedshelflifeis12monthsfromthedateofpurchase.SafetyConsiderationsTheretrovirusinfectedcellmediaandthepurifiedviruscanbepotentialbio-hazardousma