《文献组会》PPT课件.ppt

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1、α‑GlucosidaseInhibitorsfromPreussiaminimoidesRangelgrimaldoM,RiverocruzI,MadariagamazónA,etal.α-GlucosidaseInhibitorsfromPreussiaminimoides[J].JournalofNaturalProducts,2016..................................................................................

2、...............................................................................................................................................................................AbstractExperimentConclusionMyopinion1234AbstractIsolatetwonewpolyketideswithno

3、velskeletons,minimoidionesA(1)andB(2)withthreeknowncompounds.Deducetheabsoluteconfigurationby1Dand2DNMR,ECD,DFT,single-crystalX-raydiffractionanalysis.α-glucosidaseexperimentIsolationandIdentificationofP.minimoidesFermentation,Extraction,andIsolationCrud

4、eextract38ug/mlminimoidionesA(1)minimoidionesB(2)1D和2DNMR,ECD,Single-crystalX-raydiffractionanalysisTwopossibleenantiomersDFT7aS,8S,11aSX-rayanalysisfailedTwopossibleExperimentConclusionPartialstructuresaandbandselectedHMBCandkeyNOESYcorrelationsofminimo

5、idioneA(1).abminimoidioneA(1)ABCDABDcdminimoidioneB(2)PartialstructurescanddandselectedHMBCandkeyNOESYcorrelationsofminimoidioneB(2)ABCDEABCDECompoundIC50(µM)∆G(kcalmol-1)minimoidioneA(1)95.2±0.8-8.5minimoidioneB(2)2.9±0.47-7.7(R)-10.3(S)preussochromone

6、C(3)66.5±0.97-7.9corymbiferone(4)155.5±4.25-7.6acarbose*876.9±38.8-9.9InhibitoryEffectanddockingbindingenergies(∆G)ofCompounds1-4TestedAgainstYeastα-Glucosidase(αGHY)2DRepresentationoftheinteractionsamongαGHYandacarboseinthepredictedbindingsite.Modelofbi

7、ndingforcompounds1(yellowsticks)andthetwostereoisomersof2,R(cyansticks)andS(greensticks),withαGHY(bluecartoon,pdb3A4A).Theinsetsshowdetailsofthebindingmodefor1(AandE),2R(BandF),and2S(CandG)Thankyou