小鼠血液收集SOP.doc

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1、RodentBloodCollectiontechniquesGuidelines:TheproceduresdescribedinthisSOPshouldbefollowedwhencollectingbloodsamplesfrommice,rats,orhamsters.Bloodvolumeofanindividualanimalisapproximately6-8%ofbodyweightinadults.Upto10%ofthetotalbloodvolumecansafelyberemovedfroma

2、healthyanimalwithoutfluidreplacementattwoweekintervals.Ifalargersampleisneededatonetime,upto15%canbeslowlyremovedifIVfluidsaregiventoreplacethelostvolume.Maximumwithdrawalvolumesmustbefollowedtominimizetheriskofanemiaandcardiovascularcollapse.MaximumBloodcollect

3、ionvolumesCollectionIntervalPercentofBloodvolumePercentofbodyweightFluidreplacementrequiredOnetime15%1.5%YesEvery2weeks10%1.0%NoWeekly7.5%0.75NoTerminal30-50%3-5%N/AMouseRatHamsterAdultbodyweight25-35g250-500g90-120gAdultBloodvolume2-2.75mL15-30mL5.4-7.2mLWeekly

4、samplevolume(7.5%)0.15-0.211.125-2.25mL0.4-0.54mLEverytwoweeksampling(10%)0.2-0.28mL1.5-3.0mL0.54-0.72mLOnetimewithfluidreplacement(15%)0.3-0.42mL2.25-4.5mL0.81-1.08mLTerminal1-1.5mL8-12mL3-5mLBodyweightscanvarywithage,strain,andgender.Therefore,itisrecommendedt

5、hatanimalsareweighedtocalculatemaximumbloodcollectionvolumes.Forexperimentalgroupsofthesameage,strain,andgendertheaveragegroupweightcanbeusedtocalculatebloodcollectionlimits.KMJ/DraftedMarch2010/UpdatedSeptember,2012KGRodentBloodCollectiontechniquesMaterials:Ste

6、rileHematocrittubesSterilePasteurpipetsSterileneedles-25Gand23GSterilesyringes-1CC,3CC,5CCand10CCSterilescalpelblades-size15Clippers70%ethanolGauzeBloodcollectiontubesHeatlampTissueglueTopicalophthalmicanesthetic,suchasproparacaineOphthalmicantibioticointmentRod

7、entrestrainerRatrestraintboardforjugularbloodcollectionwithattachedlegrestraintropesPlasticnoseconeforheadrestraintProceduresinthemousecangenerallybeperformedbyoneperson.Forratsandhamsterssometechniquesmayrequiretwopeople,onetorestraintheanimalandonetocollectthe

8、blood.Otherprocedureswillrequirearestraintdeviceorlightanesthesia.Terminalbloodcollectionbycardiacpuncturecanonlybeperformedunderanesthesia.Methods:Tailnick:Thisproce