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1、cmh毕业论文终稿毕业论文 由本实验室构建的猪伪狂犬病毒基因缺失株PRVTK-/gE-/LacZ+作为一种病毒载体，具有安全性好、容量大、重组效率高等优点，本研究用人工合成的O型口蹄疫P1基因作为抗原基因，用和泛素（Ub）融合的UbiP1作为另一个抗原基因同时达到增强细胞免疫效果，再将针对口蹄疫3B基因设计的shRNA和具有抗病毒及免疫调节效果的猪IFN-γ与以上两个功能基因串联，构建了具有四个功能基因的转移载体，将此转移载体和PRV缺失株TK-/gE-/LacZ+共转染细胞，发生基因重组后进行了空斑纯化，经鉴定，初步得到了含有
2、P1基因、UbiP 1、shRNA、IFN-γ四个功能基因的重组伪狂犬病毒，为进一步构建新型基因工程疫苗打下基础。 关键词抗原基因；泛素蛋白基因；干扰素基因；重组病毒AbstractFootandmouthdisease(FMD)isanacute,highlycontagiousvirulinfectiondisease.Althoughvaines,availablesincetheearly1900s,havebeenusedinpartsoftheworld,thediseasestillisaseriousglobe
3、outbtreakandbeesanitarybarriertothemerceofanimalsandanimalproducts.ThevectorvirusPRVTK-/gE-/LacZ+,constructedbyourlaboratory,hastheadvantagesofsafety,highcapacity,highrestructuringefficiency.ThisstudyweselectesyntheticOtypeFMDVP1geneasantigengene,P1andubiquitin(Ub)fus
4、iongeneUbiP1asanotherantigengeoenhancecellularimmuneeffect.ThenuseshRNAdesignedfor3BgeneofFMD,IFN-γgeneofporcinehasanti-viralandimmunomodulatoryeffectstandemwiththeabovetwofunctiongene,constructedtransfervectorwithfourfunctionalgene.MakingthetransfervectorandthePRVTK-
5、/gE-/LacZ+cotransfectedincells.Aftertheourrenceofrebinant,plaqueswerepurified,identified,initiallyreceivedrebinantpseudorabiesviruswithfourfunctionalgenesofP1gene,UbiP1,shRNAandIFN-γ,inordertofurtherbuildanewfoundationfortheconstructionofgeicengineeringvaine.Keywordsa
6、ntigengene;ubiquitin;interferongene;rebinantvirus专业文档，值得下载！专业文档，值得下载！英文缩略词表Abbreviation缩写FMDFMDVUbUPSRNAisiRNAshRNASIFIFNγPRVNCSAmpDMEMLBPBSPCRSDSEBODEDTAPKVeroDMSOMEM英文名称Foot-and-monthdiseaseFoot-and-MouthdiseasevirusUbiquitinUbiquitin-proteasomesystemRNAinterference
7、RNAinterferencegeneRNAinterferencegeneInterferencegenefragmentγ-interferongenePseudorabiesvirusNewborncalfserumAmplicillumDulbeo'sModifiedEagleMediumLuria-BertanimediumPhosphatebuffersolutionPolymerasechainreactionSodiumdodecylsulphateEthidiumbromideOpticaldensityEDTA
8、DisodiumSaltPigkidneycellsAfricangreenmonkeykidneycellsDimethylsulfoxideMinimalEssentialMedium中文名称口蹄疫口蹄疫病毒泛素泛素蛋白酶系统小RNA干扰小RN
