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1、TheresearchaboutREICexpressionanditscorrelationwithclinicopathologicalparametersofgastriccarcinomasXuXiaoyam,XiaP112,ZhengHuachuan?(1.DepartmentofPathophysiology,InstituteofPathologyandPathophysiology,CollegeofBasicMedicine,ChinaMedicalUniversity,ShenYang1100
2、01;2.DepartmentofBiochemistryandMolecularBiology,InstituteofPathologyandPathophysiology,CollegeofBasicMedicine,ChinaMedicalUniversity,ShenYang110001)Abstract:REICisdown-rcgulatcdinimmortalizedcelllinescomparedwiththeparentalnormalcounterparts.Itmayinhibitcolo
3、nyformation,tumorgrowth：andinducetheapoptosis・Here,REICexpressionwasexaminedingastriccarcinomasbyRT-PCR,Westernblotandimmunohistochemistry,andcomparedwithclinicopathologicalparametersofcarcinoma・Immunohistochcmically,REICexpressionwasnegativelyassociatedwitht
4、umorsize,lymphnodemetastasis,dcdiffcrcntiationorpoorprognosisofcarcinoma.TheseresultssuggestthatREICexpressionmaybeapromisingobjectiveandeffectivemarkertopredictpathobiologicalbehaviorsandprognosisofgastriccarcinoma.Keywords:gastriccarcinoma;REIC;prognosis0In
5、troductionGastriccancerisrankedasthefourthmostcommonandthesecondmostfrequentcauseofdeathfromcancerbecauseoftheslowdecreaseinincidenceinAsiaandhighmortalityfromdiagnosedgastriccarcinomasintheWest,eventhoughadvanceddiagnosticandoperativetechniquesarewidelyappli
6、edinclinicalpractice[1,2].Tumorigenesisandprogressionofgastriccarcinomaisamultistageandmultifactorialprocess,henceincreasedunderstandingofchangesingeneexpressionduringcarcinogenesis,particularlyidentificationofnovelbiomarkersforcancerdiagnosisandnoveltherapeu
7、tictargetswillimprovediagnosis,treatmentandprevention.REIC(ReducedExpressioninImmortalizedCells)wasisolatedbytherepresentationaldifferenceanalysisbecauseitsexpressionisdown-rcgulatcdinimmortalizedcelllinescomparedwiththeirparentalnormalcounterparts・Ahomologys
8、earchrevealsREICisidenticalwiththehumanDkk-3gene・ThehumanREICeDNAencodesasecretedglycoproteinof350aminoacidswithfourpotentialN-glycosylationsites,includinganN-terminalsignalpeptide,twocys
