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1、WuhanUniversityTheCollegeofLifeScienceTheReplicationofDNAContents13InitiationofDNAReplication4ElongationofDNAReplication5FinishingofDNAReplication6TheConceptsofDNAReplicationEnzymeandProteinInvolvedinReplication2MaterialsAndSpecialConstructureofDNAWWW.SOPPT.CNTheConc
2、eptsofDNAReplication●DNAreplicationisaprocessinwhichtwosamecopiescanbeobtained.●DNAreplicationissemi-conservative,onestrandservesasthetemplateforthesecondstrand.●Furthermore,DNAreplicationonlyoccursataspecificstepinthecellcycle.WWW.SOPPT.CNMaterials●Template:Oneofthe
3、strandofdsDNA;●dNTPs:4kinbsofdeoxynucleotidetriphosphates,that's,ATP,GTP,CTP,TTP;●Primer:usuallyRNA;●Proteinsandenzyme:suchasDNApolymerase,DNAhelicase,SinglestrandDNAbindingprotein（SSB）,DNAtopoisomerase,DNAligase,Telomerase(specialineuka-ryotes);WWW.SOPPT.CNSpecialCo
4、nstructureofDNADuringReplicationTheentiresetofcis-actingDNAsequencesthatissufficienttodirecttheinitiationofDNAreplicationDistinctionbetweenthereplicatorandoriginofre-plication-------Originofreplicationisalwaysapartofthereplicator.ReplicatorThespecificsitesatwhichDNAu
5、nwindingandinitiationofreplicationoccur,arerequiredforDNAreplicationinitiation.It'ssequencearerichinAT,usuallyisacrossst-ructureoriginWWW.SOPPT.CNSpecialConstructureofDNADuringReplicationRepliconAsaunitforreplicationaDNAsequence.Itcont-ainsareplicationorigin,sometime
6、sitalsocontainsafinishingsequence.EukaryoticrepliconProtokaryoticrepliconWWW.SOPPT.CNSpecialConstructureofDNADuringReplicationReplicationForkIt'stheYshapeareaformedbydsDNAwhichisbeingreplicated.UnwindedtwosingletemplateDNAandthenewchainswhichisbeingsynthetizedconsist
7、oftheheadofY,anddsDNAtemplateconstitutesthetailofY.WWW.SOPPT.CNSpecialConstructureofDNADuringReplicationOkazakifragmentInDNAdiscontinuousreproduction,it'stheDNAfragmentssynth-etizedusingthelaggingstrandtemplate.ThentheyarecovalentconnectedtoassDNA.Itslengthisdifferen
8、tinprokaryoteandeukaryote.WWW.SOPPT.CNEnzymeandProteinInvolvedinReplication●DNApolymerase;●DNAhelicase;●SinglestrandDNAbindingprote
