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1、孙志远丁仕义黄学全【摘要】目的利用兔VX2川:移植瘤模型探讨高浓度乙酸注射治疗肝肿瘤的价值，并探索注射治疗肝肿瘤的最适乙酸浓度。方法用移植法建立兔VX2肝肿瘤模型，将40只携瘤兔随机分为A、B、C、D四纽。术前以CT测量肿瘤直径。开腹向A、B、C、D组肝肿瘤内分别注入50%、75%、100%乙酸和生理盐水,48h后重复注射一次,术示一周处死兔了取肝脏观测比较各纟II肿瘤体积改变情况及坏死灶周围新生灶悄况，并取肿瘤组织制作石蜡切片(HE染色)，镜下观察肿瘤组织坏死情况。结果注射后一周各实验组肿瘤直径均减小，对照组肿瘤直径明显增大。100%乙酸组10例仅1例在坏死灶周围发现新生灶
2、，而50%乙酸组发现5例,75%乙酸组发现7例。镜下显示100%乙酸组肿瘤中未坏死的肿瘤细胞团较75%和50%乙酸组少见。结论高浓度的乙酸对兔VX2肝肿瘤的灭活效果显箸;其中100%的乙酸效來最佳。【关键词】肝肿瘤乙酸疾病模型，动物兔AceticacidinjectionforthetreatmentofVX2livertumorbydifferentconcentrationofaceticacid:ancx2pcrimcntalstudyinrabbitSunZhiyuan,DingShiyi,HuangXucquan.DepartmentofRadiology,Sout
3、hwestHospitai,ThirdMinitaryMedicalUniversity,Chongqing400038,China[Abstract]ObjectiveTostudythevalueofaceticacidinjectionforthetreatmentoflivertumor,andexplorethebestconcentrationofaceticacid.Methods40rabbitswithimplantedVX2liv2ertumorwererandomlyweredividedinto4groups.Beforethetreatment,th
4、ediameterofthetumorswasacquiredbyCT.Theabdomenwasopened,and50%,75%,100%aceticacidorphysiologicsa21inewasinjectedintothetumorsofA,B,C,Dgroups,respectively.After48hoursaceticacidwereinjectedonceatthesamedosage.Afteraweektherabbitswerekilledandtheliverswereresected.Thetumorsdiameterweredetermi
5、nedandthecasesoftherenascenttumorswerecountedineachgroup.Sometumortissuesweresampledmakeparaffinsection(HEstaining)andobservethenccrosisoftumortissuewithmicroscope.Results1weekafterinjection,theaveragediameteroftumorsineachtestgroupdecreasedwhencomparedwiththecontrolgroup.In100%aceticacid2g
6、roup,onlyleasehadrenascenttumorsnearthenecrosisfocus.In75%aceticacid2group,5caseshadrenascenttumors,andin50%aceticacid2group,7caseshadrenascenttumors.Undermicroscope,thesurvivaltumorcellsin100%aceticacid2teamwerefewerthanin50%aceticacidand75%aceticacid2group.ConclusionsTheeffectofhighconcen
7、trationofaceticacidinjectionforthetreatmentofVX2livertumorinrabbitissignificant,and100%aceticacidhasthebesteffect.[Keywords]hepaticneoplasmsaceticaciddiseasemodels,animalrabbit经皮乙酸注射治疗(percutaneousaceticacidinjection,PAI)肝癌的方法由日本学者Ohnishi于1994年首先报道[1]。该技
