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1、破溃兔扩张皮瓣细菌学探究［摘要］目的：研究扩张皮瓣破溃后不同时段及部位的细状态。方法：以新西兰大白兔作为实验动物。首先形成兔扩张皮瓣模型，进而形成破溃的扩张皮瓣模型，并随机分为A、B、C、D破溃时间长短不同的四组。各组的皮瓣组织均以破口为中心，呈环状由内向外分为6个部分；然后在无菌的环境下分别对这6个部分进行标本采集。采取的标本采用Cooney法进行细菌定量检查和常规接种、需氧和厌氧培养定性检查。结果：①随着破溃时间的延长，破口周围炎症反应的皮肤长度逐渐增大，皮瓣组织内的细菌数量逐渐增多；各组间有显著差异(P
2、<0.05);②破溃时间大于3周，皮瓣基底出现感染几率明显增大(^%vs100%;Pv0・05),此时整个皮瓣组织都有细菌的存在；③皮瓣基底未感染时，细仅存在于破口周围炎症反应的皮肤包膜复合组织及其外0・5cm的范围内；④存在的细菌主要为革兰阳性菌。结论:扩张皮瓣破溃后，皮瓣各部的细菌状况会出现改变，临床上应予注意。［关键词］扩张皮瓣；破溃；细菌学m2［中图分类号JR332［文献标识码］A［文章编号］1008-6455(2012)01-0058-03Theexperimentalstudyofthebacte
3、riologyoftherecipientbedoftherupturedexpandedflapHUShou-duo1,ZHAOYan-yong2,JIANGHai-yue2,YANGQing-hua2,ZHUANGHong-xing2(1.PlasticandCosmeticSurgeryDepartmentofthePekingHospitalofIntegratedChinesewithWesternMedicine,Beijing100039,China;2.PlasticSurgeryHospit
4、alofChineseAcademyofMedicalSciences,Beijing100114,China)Abstract:ObjectiveTostudythebacteriologyoftherupturedexpandedflap.MethodsTheNewZealandWhiterabbitswereselectedasexperimentalanimals.Firstly,therupturedexpandedflapanimalmodelsweremadeandwererandomlycla
5、ssifiedintofourgroups,namedasAgroup,Bgroup,CgroupandDgroup・Thetissuesoftheflapofeachgroupweredividedintosixpartsfromcentertoedgewhentakingtheruptureasthecenter.Thespecimensweretakenfromtheseparts・AllthespecimenswerequantificationallyexaminedwithCooney'smeth
6、odandqualitativelyexaminedforgramsmearandaerobicandanaerobiccultures.ResultsTheexperimentresultsrevealedthat:©withtheextensionoftherupturetime,thelengthoftheinflammatoryreactionskin-capsulecompositetissuegraduallyincreased,thebacterialnumberintheflaptissuei
7、ncreased.thesignificantdifferencesexistedbetweeneachgroup(PD0.05).②Iftherupturetimelastedmorethanthreeweeks,theinfectiousratioofthefloorwouldincreasesignificantly(17%vs67%;PD0.05).whenthefloorinfectiondidexist,bacteriacouldbefoundinallpartsoftheflap・®lfthef
8、loordidn'tinfeet,thebacteriaexistwithinthescope0.5cmIongerthantheinflammatoryreactionskirrcapsulecompositetissues・④themainkindofbacteriawasgram-positivebacteria.ConclusionsThebacteriologyoftheflapwasch
