大鼠异种穿透性角膜移植的临床免疫学研究

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1、人鼠异种穿透性角膜移植的临床免疫学研究眼科研究200（）年第1期第18卷实验研究作者：丁伟伍桂军陈家祺杨方燿王晶刘业滋单位：丁伟伍桂军杨方耀刘业滋（广州，第一军医大学附属南方医院眼科510515）：陈家祺（中山医科大学中山眼科中心）；王晶（空军南京医院免疫室）摘要目的研究大鼠角膜移植排斥反应发生机理。方法制作模型（Wistar大鼠18只，豚鼠9只）。观察角膜透明度、新生血管。角膜和外周血行免疫检测。并对角膜进行扫描电镜观察。结果术后1周，植床出现新生血管、角膜水肿、基质增厚；术后1月，植床瘢痕化，但水肿减轻。免疫组化显示：移植后3天，血中Th/i细胞

2、IL-2R+细胞升高，15天时角膜植片内有以TC/S细胞浸润为主的淋巴细胞。结论大鼠穿透性角膜移植术后排斥反应有着与机体其它器官相似的移植排斥过程。关键词异种穿透性角膜移植免疫分类号R779.65TheclinicalimmunologystudyofcornealpenetratingxenograftinWistarratsDingWeiWuGuijunChenJiaqi（DepartmentofOphthalmology,NanfangHospitabFirstMilitaryMedicalUniversity,Guangzhou510515）

3、AbstractObjectiveToinvestigatetherejectionmechanismsofcornealpenetratingxenograft・MethodsThecornealpenetratingxenograftmodelsweremadein18Wistarratsand9guineapigs.Thediameterofgraftwas6.0mm,anddiameterofreceiptbedwas0.5mmshorterthanthegraft・Thegraftwascontinuoussuturedbynylonwir

4、e・Cornea1pellucidity,edemaandneovciscularizationwereobservedbyoptonslit-lampmicroscopyevery4d,andstoppedtill30dafterkeratoplasty.Rejectionvalue（RV）wascalculatedaccordingtoHolland"sgrade.On6,lOd（oneeyeball）and15d（twoeyeballs）afterkeratoplasty,immunohistochemicalstudywasperformed

5、inthecomea.On3dpreoperationand3,9,15,21,30dpostoperation,1.5mmbloodwasgottenoutfromtherat'shearts,andthechangesoflymphocytesubgroupwereobserved・On12,20dafterkeratoplasty,thecornealconstitutionandformoftheanimalswererespectivelyobservedandtakenaphotographbyscanningelectronmicros

6、cope.Immunologicalstudywasperformedinthecorneaandtheperipheralblood.ResultsInthefirstweekofpostoperation,neovascularization,cornealedemaandthicknessofstromaoccurredinrecipientsite.Inthefirstpostoperativemonth,therecipientsitecicatrizedandcornealedemalessened.Immunohistochemical

7、studiesshowedthatTh/icellsandIL-2R+cellswereincreasedintheperipheralbloodon3dafterkeratoplasty,At15dafterkeratoplastytthemajorinfiltratingcellswereTc/scellsinthegraftcorneas.ConclusionTherejectionofcornealpenetratingxenograftinratsmaybesimilartothatofotherorgans.KeywordscorneaI

8、penetratingxenograftimmunologicalstudy眼外伤和感染性角膜疾病均可导致角