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1、以Fe(III)为电子受体聚磷菌筛选、鉴定及聚磷特性作者简介：任丽平(1971),女，副教授，博士生，主要从事资源微生物和水污染控制研究，(Email)xhrlph@163.com0张智(通信作者)，男，教授，博士生导师，(Email)zhangzhicq@126.com0摘要：结合平板分离法、poly-P和PHB染色以及柠檬酸铁还原实验，从污水处理厂活性污泥中分离获得一株以Fe(III)为电子受体厌氧聚磷的聚磷菌株AP3,其16SrDNA与PseudomonasmosseliiATCCBAA-99的同源性为99%。菌株AP3于柠檬酸铁液体培养基和无铁液体培养基中厌氧避光
2、、27°C静置培养，在含有Fe(III)的培养基中比在无Fe(III)的培养基中生长好；菌株AP3在无Fe(III)的培养基中，表现出典型的厌氧释磷特征，释磷量为3.68mg•L-1；在含有Fe(III)的培养基中，先厌氧释磷，释磷量为6.62mg•L-1,10h之后开始厌氧聚磷,聚磷量为5.89mg•L-lo菌株AP3能以Fe(III)为电子受体,具有厌氧聚磷特征。关键词：聚磷菌；筛选；鉴定；氧化铁；电子受体中图分类号：X172文献标志码：A文章编号：16744764(2012)03014605ScreeningandIdentificationofPhosphateA
3、ccumulatingBacteriawithFe(III)asElectronicAcceptoranditsEffectsonPhosphorusAccumulatingCharacteristicsRENLipingl,2,ZHANGZhil,TANGYun2(1.CollegeofUrbanConstruetionandEnvironmentalEngineering,ChongqingUniversity,Chongqing400045,P.R.China；2.CollegeofBiologicalSciences,ChinaWestNormalUniversi
4、ty,Nanchong637002,P.R.China)Abstract：AphosphorusaccumulationorganismstrainAP3withFe(III)aselectronicacceptorwasscreenedfromactivatedsludgethroughsuchprocessesasdilutionplatemethod,polyP,PHBstainingandferriccitratereduction.Its16SrDNAisidenticaltothatofPseudomonasmosseliispATCCBAA99at99%le
5、vel.BycomparingtheAP3beingculturedinferriccitratefluidmediumandtheoneinnonferricfluidmediumat27°C,itwasfoundthatferriccitratefluidmediumwasbeneficialfortheAP3・AP3culturedinnonferricfluidmediumshowedsignificantanaerobicandphosphorusreleasingcharacteristic,thephosphorusreleasingamountofwhic
6、hwas3.68mg•L—l.AP3culturedinfeiricfluidmediumwasalsocharacterizedbyanaerobicandphosphorusreleasingpropertyatthefirststage,thephosphorusreleasingamountofwhichwas662mg•LT,whichwashigherthantheformer；AndatthesecondstagewhichstartedfromthelOh,itwascharacterizedbyanaerobicandphosphorusaccumula
7、tingproperty,thephosphorusaccumulatingamountofwhichwas5.89mg•L~1.Fe(III)istheelectronacceptorofAP3whichshowstypicalanaerobicandphosphorusabsorbingproperty.Keywords:phosphorusaccumulationorganism；screening；identification；ironoxides；electronicacceptor现代污水生物强化除磷法是通过聚磷菌
