人氟斑牙早期龋再矿化的初步研究

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1、人氟斑牙早期鹉再矿化的初步研究人氟斑牙早期舗再矿化的初步研究作者：罗丽娅王永黎红郑辉高思继作者单位：1•口腔疾病研究国家重点实验室，四川大学，四川成都610041；2•贵阳医学院附属医院口腔科，贵州贵阳550004；3•遵义市口腔医院口腔内科，贵州遵义563000【摘要】目的研究离体氟斑牙早期舗模型经再矿化处理后，釉质表面显微硬度及微观形貌的变化。方法应用离体氟斑牙制备釉质样本40个，经酸蚀凝胶人工鵠实验脱矿后，1.0%酪蛋白磷酸肽■磷酸钙(CPP-CP)再矿化液再矿化。采用显微硬度计测定各样本脱矿前后及再矿化后

2、的显微硬度值。采用体视显微镜、偏光显微镜、透射显微镜以及扫描电镜观察釉质表面微观形貌。结果脱矿前，釉质表面显微硬度值为(241.53±21・31)kHV；脱矿后，表面显微硬度下降到(175.76±24.99)kHV(P<0・05)。其表面硬度损失百分比(％SMHD)为(27.23±4・79)%,表现为类似早期舸的表层下脱矿；再矿化后，表面显微硬度提高到(210.17±21.48)kHV(P<0.05),其表面显微硬度恢复的百分比(%SMHR)为(52.32±4.23)%,主要表现为表层下再矿化。结论再矿化处理对治

3、疗氟斑牙早期舗有较好的临床效果。【关键词】氟斑牙再矿化显微硬度StudyonremineralizationofhumanfluorosedteethinvitroLUOLi-yal,WANGYong2,LIHongl,ZHENGHu订，GAOSi-ji3・(1・StateKeyLaboratoryofOralDiseases,SichuanUniversity,Chengdu610041,China;2.Dept,ofStomatology,TheAffiliatedStomatologicalHospital

4、ofGuiyangMedicalUniversity,Guiyang550004,China;3.Dep匸ofOralMedicine,TheStomatologicalHospitalofZunyi,Zunyi563000,China)[Abstract]ObjectiveToinvestigatethetransformationofmicrohardnessandmicrostructuresofhumandentalflu-orosisafterdemineralizationandremineraliz

5、ationinvitro.MethodsFortyhumandentalfluorosisenamelblocksweredemineralizedbyacid-etchinggel,thensubjectedto1.0%caseinphosphopeptidesstabilizedcalciumphosphate(CPP-CP)forremineralization.Surfaceenamelmicrohardnesswasmeasuredontheenamelblocksbeforeandafterdemin

6、eralizationandafterremineralization.Theenamelspecimenswasobservedbystereomicroscopeandscanningelectronmicroscope,thensectioned(100Mm)andexaminedbytransmissionlightmicroscopeandpolarizedlightmicroscope・ResultsBeforedemineralization,theaverageknoophardnessvalue

7、ofthefluorosedteethwas(241.53±21.31)kHV.Afterdemineralization,obviousdecreaseofthesurfacehardnessoftheenamelwasobserved,themeanvaluewas(175.76±24.99)kHV(P<0.05),thepercentofthesurfacemicrohardnessdemineralization(%SMHD)was(27.23+4.79)%,andmajordemineralizatio

8、nwasunderthesurfaceoftheenamel,whichwassimilartotheearlynaturalenamelcaries・Afterremineralization,obviousincreaseofthesurfacehardnessoftheenamelwasobserved,themeanvaluewas(210.17+21.48)kH