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1、1.按文中意见修改后在改，2.短芽抱杆菌3.要有枯萎病抑制图，4.要有测序图5.取消DNA图6.不耍用20%三醴酗作物枯萎病尖胞镰刀菌拮抗菌株JK-2的筛选和鉴定葛慈斌",郝晓娟X,黄素芳I,朱育菁I,郑雪芳I,刘波"(1.福建省农业科学院生物技术研究所生物农药研究中心，福州350003;2.浙江大学农业与生物技术学院，杭州310029)摘要：从福建省永泰、福淸、闽侯等6个县(市)采集作物根际土壤，筛选出芽抱杆菌58株。采用目标病原菌多垂菌株平行测定法，试验初筛出的芽抱杆菌对6株枯萎病尖他镰刀菌的抑制作用，筛选得到拮抗菌株3株，其中
2、1株(定名为JK・2)的抑菌效果特别显著。对JK・2的抑菌谱调查表明,该菌株对大丽轮枝菌(VerticiIliumdahliae')^黑白轮枝菌(VerticiIliumalbo-atrum)s真菌轮枝得(VerticiIliumfungicola鳄梨根腐病原菌(VerliciUiumfheobromae)、青枯雷尔氏菌(Raistoniasolanacearum)和禽致病性人肠杆菌(Escherichiacoli)都具冇较强的抑制作用。对JK-2进行菌体形态、培养特征观察及生理生化试验，再结合16SrDNA序列测定及Sherlo
3、ckMIS脂肪酸分析，判定该菌株为fel芽他杆菌(Brevibacillusbrevis)o将JK-2培养液浇灌移栽后1天的西瓜苗，试验其川间防治西瓜枯萎病的效果，1个月后，防效可达78.26%。关键词：生防菌；尖抱镰刀菌；短芽砲杆菌；SherlockMIS脂肪酸分析中图分类号：文献标识码：AScreeningandIdentificationofBiocontrolBacteriaStrainJK-2againstPlantPathogenicFungus,FusariumoxysporumSchl.LIUBo1,GECi-bin
4、1,ZHUYu-jing1,ZhengXue-fang1,HaoXiao-juan1,2,HuangSu-fang1(1BiocideCenter,InstituteofBiotcchonology,FAAS,Fuzhou350003,China;2.1nstituteofBiotechonology,UniversityofZhejiang,Hanzhou310027,China)Abstract:Fifty-eightstrainsofBacillusspp.wereisolatedfromtherhizospheresoils
5、,whichwerecollectedfromYongtai,Fuqing,Minhouandother3countiesinFujianProvince.Usingmultipletargetpathogensinhibitingtestwith6strainsofFusariumoxysporumSchl.,threestrainsofbiocontrolbacteriawerescreenedtoshowhighinhibitionagainstF.oxysporum•Amongthesestrains,onestrain,w
6、hichwasnamedwithJK-2,wasthemosteffective・JK-2hadstrongantagonisticeffectonabroadspectiumofpathogenicfungiandbacteria,suchasVerticilliumdahliae,Verticilliumalho-atruin,Verticilliumfim^icola,Verticilliumtheohromae,RaistoniasolanacearumandEscherichiacoli.Basedontheobserva
7、tionsonitsmorphologicalfeaturesandculturecharacters,aseriesofphysiologicalandbiochemicalexperiments,SherlockMISanalysisandits16SrDNAsequencephylogeneticrelation,JK-2wasidentifiedasBrevihacillusbrevis.ThebioassayinfieldindicatedthatJK-2reducedwatermelonfusariumwiltsever
8、ityby78.26%onemonthafterinoculatingJK-2intotherhizospheresofwatermelons,whichwereplantedonedaybefore・基金项比国家863计划项hl(2
