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1、Method1681:FecalColiformsinSewageSludge(Biosolids)byMultiple-TubeFermentationusingA-1mediumJune2005U.S.EnvironmentalProtectionAgencyOfficeofWater(4303T)1200PennsylvaniaAvenue,NWWashington,DC20460EPA-821-R-04-027AcknowledgmentsThisreportwaspreparedbytheDynCorp/CS
2、CBiologyStudiesGroupunderthedirectionofRobinK.Oshiro,oftheOfficeofScienceandTechnology’sEngineeringandAnalysisDivisionwithintheU.S.EnvironmentalProtectionAgency(EPA’s)OfficeofWater.ThisreportwaspreparedinsupportofaprojectleadbyMarkC.MeckesoftheEPAOfficeofResearc
3、handDevelopment’sNationalRiskManagementResearchLaboratory(NRMRL).Thecontributionsofthefollowingpersonsandorganizationstothisstudyaregratefullyacknowledged:RefereeLaboratory•EPAOfficeofResearchandDevelopment,NationalRiskManagementResearchLab:MarkC.MeckesandKarenM
4、.WhiteVolunteerParticipantLaboratories•AmericanInterplex:JohnOverbeyandLizbethHuggins•BioVirLaboratories:RickDanielsonandJimTruscott•CityofLosAngelesBureauofSanitationEnvironmentalMonitoringDivision:FarhanaMohamedandZoraBahariance•CountySanitationDistrictsofLosA
5、ngelesCounty:ShawnThompsonandJulieMillenbach•EnvironmentalAssociates:SusanBoutrosandJohnChandler•HamptonRoadsSanitationDistrict:AnnaRuleandBobMaunz•KingCountyEnvironmentalLaboratory:GregMaandBobbieAnderson•HoosierMicrobiologicalLaboratories:DonHendrickson,KeriNi
6、xon,KatyBilger,andLindseyShelton•MassachusettsWaterResourcesAuthority:SteveRhodeandMariyaGofshteyn•MilwaukeeMetropolitanSewerageDistrict:JeffMacDonaldandTimO'Neill•UniversityofIowaHygienicLaboratory:NancyHallandCathyLord•UtahDepartmentofHealth:SanwatChaudhuriand
7、DevonColeThefollowingfacilitiesprovidedbiosolidmatricesforthestudy:•CompostFacility,Columbus,OH:AngelaBianco•WastewaterTreatmentFacility,SturgeonBay,WI:ToddMaurina•WastewaterTreatmentFacility,Fairfield,OH:DrewYoung•WastewaterTreatmentFacility,Mason,OH:ErnieStick
8、ler•N-ViroTreatmentFacility,Toledo,OH:CindyDrillMediaPhotographs•MarkC.Meckes,NRMRL,USEPAiiiDisclaimerThismethodisindraftform.IthasnotbeenreleasedbytheU.S.Environment
