微柱凝胶技术检测血型不规则抗体的临床应用

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1、微柱凝胶技术检测血型不规则抗体的临床应用［摘要］目的分析微柱凝胶技术对输血患者进行血型不规则抗体的筛查情况及临床应用。方法对2010年1月~2013年12月于桂林医学院附属医院输血患者29208例进行不规则抗体检测，统计分析阳性结果，鉴定阳性抗体的特异性。结果29208例患者中，检出不规则抗体阳性者90例，阳性率为0.31%0特异性抗体以Rh血型系统居多，抗-D抗体占2.22%.抗-E抗体占21.11%、抗-Ec抗体占11.11%；MNSs系统抗-Mur抗体11例(12.22%)、抗-M抗体7例(7.78%)；Lewis系统抗-Lea抗体占8.

2、89%；冷抗体占7.78%o非特异性抗体占13.33%O结论输血前对患者血液进行血型不规则抗体筛查并鉴定其特异性，输注不含相应抗原的血液，避免溶血性输血反应的发生，对确保输血安全有效有重要的临床意义。［关键词］微柱凝胶技术；不规则抗体筛查；安全输血；输血反应［中图分类号］R457.1［文献标识码］A［文章编号］1673-7210(2014)06(a)-0091-03TheclinicalapplicationofirregularantibodyscreeningbymicrotubegeltechnologyNINGFangSUMingliD

3、ENGMeiyingADepartmentofTransfusion,AffiliatedHospitalofGuilinMedicalUniversity,GuangxiZhuangAutonomousRegion,Guilin541001,China[Abstract]ObjectiveToanalyzethesituationandclinicalapplicationofmicrotubegeltechnology(MGT)intheirregularantibodyscreeningofpatientsbeforebloodtrans

4、fusion.Methods29208patientsfromJanuary2010toDecember2013inAffiliatedHospitalofGuilinMedicalUniversitywerescreenedforirregularantibody.Theresultswerestatisticalanalyzedtoidentifythespecificityofpositiveantibody.ResultsAmong29208patients,therewere90caseswithirregularantibody,t

5、hepositiveratewas0.31%・Rhbloodtypesystemaccountedforthemostpartofthespecificantibodies:2.22%foranti-Dantibody,21.11%ofanti~EantibodyandIL11%ofanti-Ecantibody.11casesofAnti-MurofMNSssystemaccountedfor12.22%ofthetotaland7casesofanti~Mantibodyaccountedfor7.78%.8.89%wasforAnti-L

6、eaantibodyinLewissystemand7.78%forcoldantibody.Andthenon-specificantibodiesaccountedfor13.33%.ConclusionIrregularantibodyshouldbescreenedanditsspecificityshouldalsobeidentifiedbeforetransfusion・Totransfusethebloodwithoutthecorrespondingantibodiescanpreventtransfusionalhemoly

7、ticreaction,whichisofgreatsignificanceinsafeandeffectivetransfusioninclinicalwork.[Keywords]Mierotubegeltechnology;Irregularantibodyscreening;Safetransfusionblood;Bloodtransfusionreaction不规则抗体是指不符合ABO血型系统Landstemer法则的血型抗体，即抗-A、抗和抗-AB以外的血型抗体，此抗体常因输血或妊娠等免疫刺激产生，多为IgG抗体，是引起免疫性输血

8、反应、新生儿溶血病、血型鉴定困难及疑难配血（型）等的主要原因Z-o因此，输血前不规则抗体筛查和鉴定可减少因血型不合引起的输血反应，确保安全用血。特别对