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1、44.1.25A1mL/min;(4)pulsedamperometricdetectorwithgoldworkingAOACOfficialMethod2000.17electrode,i.e.,DionexPAD-2,DionexPED-2,ESACoulochemIIDeterminationofTraceGlucoseandFructose(ESA,Inc.,22AlphaRd,Chelmsford,MA01824,USA),WatersinRawCaneSugar464(WatersCorp.,34MapleSt,Milford,MA01757,USA),orHigh-P
2、erformanceAnion-ExchangeChromatographyequivalent.SettingsprovidedbythemanufactureroroptimizedbyFirstAction2000theusershouldbeused.Thesensitivity(andthustheselectedmea-[Applicabletothedeterminationofglucoseandfructosesuringrange)isaffectedbytheareaofthegoldelectrode.Typicaldi-(0.080–0.25%)inrawc
3、anesugar].ametersavailableare1.4or3mm;ameasuringrangeof1µAisSeeTables2000.17Aand2000.17Bfortheresultsofthesuitablefortheformerand5µAforthelatter.Typicalsettingsfor2interlaboratorystudysupportingacceptanceofthemethod.commonlyuseddetectorsarelistedinTable2000.17C.(b)Columnanddetectorheater.—Contr
4、olledbythermostatsetA.Principleatca27°C.Testportionandinternalstandardaredissolvedinwaterandfil-(c)Integrator.—Electronicintegrator(e.g.,HP3396,Agilentteredthrougha0.45µmmembranefilter.Thefilteredsolutionisin-Technologies,2850CentervilleRd,Wilmington,DE19808,USA)jectedontoaliquidchromatographic
5、(LC)system.CarbohydratesoraPCwithsuitablesoftware(e.g.,EzchromforWindows),oper-areseparatedonapellicularanion-exchangecolumnandmeasuredatedinthepeakheightmode.Note:Thepeakwidth,thresholdset-bypulsedamperometricdetection(PAD).Carbohydratesareidenti-tings,andotherintegrationoptionsshouldbechosent
6、oensurethatfiedandquantified(peakheightresponse)bycomparisonwithstan-thesametypeofintegrationisusedforthesugarpeaksindardsolutions.Theseparationmechanismofthesecolumnsreliesonchromatogramsofboththetestsamplesandstandards.PeakheightthefactthatcarbohydratesareweakacidsthatareionicathighpH.quantif
7、icationhasgreaterfreedomfrominterferencebyadjacentWithaneluantattheappropriatepH,manysugarscanbeseparatedpeaks,butthebaselineselectionmustbecarefullycontrolledtoen-rapidlybyhigh-performanceionchromatography.Thepellicularsureunifor
