AOAC Official Method 2000.06 Salmonella

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时间:2019-10-17

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1、17.9.27(f)Waterbath.—48–50°C.AOACOfficialMethod2000.06(g)Waterbaths.—Circulating,thermostaticallycontrolled,SalmonellainFoods43±0.2°C,and42±0.2°C.withaLowMicrobialLoad,Detection(h)Sterilespoons.—Orotherappropriateinstrumentsfortrans-FirstAction2000ferringfood.(i)Sterilegl

2、assware.—Culturedishes,15´100mm;glassor(ApplicableforthedetectionofSalmonellainfoodswithalowmi-plasticsterilepipets,1mL,with0.01mLgraduations;and5andcrobialload.)10mL,with0.1mLgraduations.(j)Inoculatingneedleandinoculatingloop.—Ca3mmidorCaution:Potassiumcyanide(KCN)brothi

3、sextremelypoison-ous.Dehydratedselenitecystine(SC)brothisvery10mL,nichrome,platinum-iridium,chromelwire,orsterileplastic.toxicifinhaledorswallowed.SCbrothisverytoxicif(k)Steriletestorculturetubes.—16´150mmandswallowed.20´150mm;serologicaltubes,10´75mmor13´100mm.(l)Testorc

4、ulturetuberacks.SeeTables2000.06AandBfortheresultsoftheinter-and(m)Vortexmixer.intralaboratorystudiessupportingacceptanceofthemethod.(n)Sterileshears,largescissors,scalpel,andforceps.(o)Lamp.—Forobservingserologicalreactions.A.Principle(p)FisherorBunsenburner.Rappaport-Va

5、ssiliadismediumandtetrathionatebrothareselec-(q)pHmeter.tiveenrichmentmediathatallowresuscitatedSalmonellatomulti-(r)Magneticstirrer.plytodiscerniblelevelsinthepresenceofcompetingmicroflora.Theincubatedmediaarestreakedontobismuthsulfite,Hektoenen-C.ReagentsandMediateric,a

6、ndxyloselysinedesoxycholateagarplateswhere,duringin-(a)Trypticase(tryptic)soybroth.—967.25A(t)(17.9.01).cubation,discreteSalmonellacoloniesareformed.Colonygrowthis(b)pHtestpaper.—967.25B(1)(17.9.01).identifiedasSalmonellawithbiochemicalandserologicaltesting.(c)Sodiumhydro

7、xide.—1MNaOH,967.25B(c)(17.9.01).(d)Hydrochloricacid.—1MHCl,967.25B(d)(17.9.01).B.Apparatus(e)Lactosebroth.—967.25A(a)(17.9.01).(a)Blender.—Calibrated,10000–12000rpm,withsterile(f)Brilliantgreendyewater.—967.25A(w)(17.9.01).blenderjars.(g)Cellulasesolution.—1%.967.25B(p)(

8、17.9.01).Add1.0g(b)Glassware.—Sterile,16oz.(500mL)wide-mouth,cellulaseto99mLHOinasterilecontaine

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《AOAC Official Method 2000.06 Salmonella》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库

1、17.9.27(f)Waterbath.—48–50°C.AOACOfficialMethod2000.06(g)Waterbaths.—Circulating,thermostaticallycontrolled,SalmonellainFoods43±0.2°C,and42±0.2°C.withaLowMicrobialLoad,Detection(h)Sterilespoons.—Orotherappropriateinstrumentsfortrans-FirstAction2000ferringfood.(i)Sterilegl

2、assware.—Culturedishes,15´100mm;glassor(ApplicableforthedetectionofSalmonellainfoodswithalowmi-plasticsterilepipets,1mL,with0.01mLgraduations;and5andcrobialload.)10mL,with0.1mLgraduations.(j)Inoculatingneedleandinoculatingloop.—Ca3mmidorCaution:Potassiumcyanide(KCN)brothi

3、sextremelypoison-ous.Dehydratedselenitecystine(SC)brothisvery10mL,nichrome,platinum-iridium,chromelwire,orsterileplastic.toxicifinhaledorswallowed.SCbrothisverytoxicif(k)Steriletestorculturetubes.—16´150mmandswallowed.20´150mm;serologicaltubes,10´75mmor13´100mm.(l)Testorc

4、ulturetuberacks.SeeTables2000.06AandBfortheresultsoftheinter-and(m)Vortexmixer.intralaboratorystudiessupportingacceptanceofthemethod.(n)Sterileshears,largescissors,scalpel,andforceps.(o)Lamp.—Forobservingserologicalreactions.A.Principle(p)FisherorBunsenburner.Rappaport-Va

5、ssiliadismediumandtetrathionatebrothareselec-(q)pHmeter.tiveenrichmentmediathatallowresuscitatedSalmonellatomulti-(r)Magneticstirrer.plytodiscerniblelevelsinthepresenceofcompetingmicroflora.Theincubatedmediaarestreakedontobismuthsulfite,Hektoenen-C.ReagentsandMediateric,a

6、ndxyloselysinedesoxycholateagarplateswhere,duringin-(a)Trypticase(tryptic)soybroth.—967.25A(t)(17.9.01).cubation,discreteSalmonellacoloniesareformed.Colonygrowthis(b)pHtestpaper.—967.25B(1)(17.9.01).identifiedasSalmonellawithbiochemicalandserologicaltesting.(c)Sodiumhydro

7、xide.—1MNaOH,967.25B(c)(17.9.01).(d)Hydrochloricacid.—1MHCl,967.25B(d)(17.9.01).B.Apparatus(e)Lactosebroth.—967.25A(a)(17.9.01).(a)Blender.—Calibrated,10000–12000rpm,withsterile(f)Brilliantgreendyewater.—967.25A(w)(17.9.01).blenderjars.(g)Cellulasesolution.—1%.967.25B(p)(

8、17.9.01).Add1.0g(b)Glassware.—Sterile,16oz.(500mL)wide-mouth,cellulaseto99mLHOinasterilecontaine

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