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1、石河子大学硕士学位论文脫毒马铃薯原原种栽培基质的研究与应用姓名：孔德鹏申请学位级别：硕士专业：蔬菜学指导教师：吕国华2011-06AbstractThetestisdividedintothreeparts:hightemperaturescreeningofcellulolyticbacteriaandorganicwastefermentation,virus-freeseedlingsofpotatocultivation.Thefirstpart,thehightemperaturephaseofcompostingfromthebarko
2、fisolatedcellulosedecompositionabilityofthetwohigh-temperaturemicrobialstrains.Onmicrobialgrowthrate,temperatureresistance,theabilitytosizeanddegradationoffilterpapercellulaseactivitiesweredetected.Thesecondpartofthetestwiththreefactorsandfourlevelsofmixeddesign,testdayinfourp
3、hasesduringthefermentationsubstratetemperaturemeasurementandPH,aftertheendoffermentationfermentationproductsmeasuredbulkdensity,porosity,conductivity,PHvalue,TN,TP,TK,nitrogen,phosphorus,potassiumandotherphysicalandchemicalproperties.Thethirdpartdealtwiththroughthefermentation
4、oforganicwasteandothersubstratesfortheratio,forthecultivationofpotato,growingseasonandharvestperiodsofobservedandmeasuredplantheight,stemdiameter,leafnumber,Huanmiaospeed,survivalrate,Bymeasuringthephysicalandchemicalpropertiesofmatrixandgrowthofcultivatedpotatomorphologicalin
5、dextofilterouttheoriginalseedforcultivationofthepotato-specificsubstrate,thusservingthepotatoplantproduction,whileachievingorganicwastetreatmentresources・Theresultsshowthat:(1)Thehightemperaturephaseofcompostingfromthebarkofisolatedcellulosedecompositionabilityofthetwohigh-tem
6、peraturemicrobialstrains・YPandNP.Growthbystrainrate,temperatureresistanceandthedetectionofcellulaseactivitywasfound,whichcanbe45〜65°Ctemperaturegrowth,optimumgrowthtemperaturewas50°C;inthegeneralbacterialculturemediumandcarboxymethylfiberSodium(CMC)assolecarbonsourcemediumarer
7、estrictedtoafaststartandstronggrowth,respectively,around84hincultureuptothemaximumnumberofviablecells・YPandNPcanformintheCongoRedmediumdistinctbrightlylitring,itscellulaseactivitytestresultsshowthattheisolateswereobtainedwithafilterpaperandhigherCxdisintegrationactivity,canthe
8、2d〜4dQuickStartroundfilterpaperdecomposition,5d〜8dcircularfil
