白念珠菌口腔黏膜感染小鼠模型的建立及评估_李成蹊

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1、白念珠菌口腔黏膜感染小鼠模型的建立及评估李成蹊，郑林霞，左露露，黄云生，陈圣琰，魏昕［摘要］目的建立白念珠菌口腔黏膜感染ICR小鼠模型，动态观察口腔黏膜及相应的全身感染情况,并对变化情况进行初步分析。方法将白念珠菌接种于免疫功能低下小鼠口腔,采用肉眼观察口腔舌背病损改变、口腔内白念珠菌菌量检测、组1天口腔白念珠菌菌量较低,随后，第4天未见明显伪膜，3〜7d内织病理学等方法评估口腔内黏膜感染情况,检测体重、载菌量(肾、肝、下降结肠内粪便)评估全身感染情况。观察1周，并记6迅速增长，3〜7d内趋于稳定，稳定于10〜QCFU/mL。同时口腔舌背病损也出现类似趋势录数据。空白对

2、照组为接种生理盐水的免疫功能低下组。结果①小鼠接种白念珠菌后第可见舌背伪膜存在。②病理切片PAS染色显示接种后3〜5d内白念珠菌形成菌丝侵入并破坏上皮，到接种第7天时，黏月崑上皮多见酵母细胞。③小鼠接种白念珠菌后体重逐渐下降。粪便载菌量逐渐上升，第5天达到最大，但肾、肝无白念珠菌感染。空白组未见白念珠菌感染。结论通过在ICR小鼠口腔内接种白念珠菌可以建立白念珠菌口腔黏膜感染动物模型。接种后白念珠菌感染程度在1周内存在变化。动物实验应根据感染状况选择合适的研究时段。［关键词］白念珠菌；口腔白念珠菌病；黏膜感染；鼠［中图分类号］R780.2［文献标识码］A［文章编号］100

3、3-98722018)05-0394-04[doi]10.1359/1j.cnki.kqyx.201&05.003EstablishmentandevaluationofamurineoralmucosalcandidiasismodelLIChengxi,ZHENGLinxia,ZUOLulu,HUANGYunsheng,CHENShengyan,WEIXin.(JiangsuKeyLaboratoryofOralDiseasesNanjingMedicalUniversity；DepartmentofEndodontics,AffiliatedHospitalofS

4、tomatology,NanjingMedicalUniversity,Nanjing210029,China)Abstract:ObjectiveToestablishamurineoralmucosalcandidiasismodel,observeoralmucosalinfectionandexaminethecorre-spondingsystemicinfectiondynamically,andtoanalyzethechangesoftheinfectionMethodsImmunosuppressedmicewereinocu・latedwithC.a

5、lbicansinfectionusingtheoralswabs.LesionsinthemurinetonguewereobservedusingmacroscopicobservationTh.eviablecellsoftheC.albicansintheoralcavitywerecalculatedandthehistologicalstudyofthemurinetongueswasanalyzedtodetermineC.albicansinfectionsoftheoralmucosalWeightandfungalburdensinthekidne

6、y,liverandfecesfromthedescendingcolonwereex・aminedtoassesstheseverityofthesystemicinfectionsTh.eaboveiindicatorsintheinfectedmicewereexaminedandrecordedforaweekafterinoculationIn.theblankgroupimmunosuppressedmicewereswabbedwithsalineResults①ThenumberofviableC.albi-6—canscellsintheoralcav

7、itywaslowonthe1stdayafterinoculation,thenrapidlygrewandtendedtobestablebetweentherangeof107CFU/mlfromday3today7afterinoculationOraltonguelesionshadasimilartrendTherewasnoobviouspseudomembranouson10day1andvisibletonguedorsalpseudomembranouscouldbeseenfromday3today7afterino