FastidiousGramNegativeRods-UNMCHome挑剔的革兰阴性杆菌-内布拉斯加大学医学中心的家庭

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1、FastidiousGramNegativeRodsBloodCultureUnitDivisionofMedicalTechnologyCarolLarsonMSEd,MT(ASCP)PleaseclickaudioicontohearCarol’snarrationFastidiousComplex/extensivenutrientrequirementsFaintstainingGramNegativeRodsSafranincounterstainfor>2minutesSubstitu

2、tecarbolfuschinforsafraninSerologicaltestingusefulClickiconforaudioGeneralInformationBrucellaspeciesClinicalSignificanceCausesBrucellosis/Undulantfever/MaltafeverTransmissionvia:DirectcontactwithinfectedanimalsIngestionofcontaminatedmeatordairyproducts

3、InhalationoftheaerosolizedorganismFacultativeintracellularorganismClickiconforaudioBlood,bonemarrowortissueRiskoflabacquiredinfectionSpecifyonrequisitionwhensuspectingBrucellaBAP,CHOC,BCYE,BloodCulturebrothClickiconforaudioBrucellaspeciesSpecimenCollec

4、tion35C,5-10%CO2for21daysColonymorphologyGrowthat7daysBAP=smoothglistening,translucentcoloniesthatbecomebrownwithageClickiconforaudioBrucellaspeciesGrowthCharacteristicsGramstain:tiny,faintstaininggramnegativecoccobacilliOxidase+,Catalase+Nitrate+

5、GlucoseoxidizerNonmotileAdditionalteststospeciateClickiconforaudioBrucellaspeciesIdentificationTubeagglutinationtestSingletiterof>=1:160FourfoldriseintiterbetweenacuteandconvalescentspecimensDoesnotdetectBrucellacanisClickiconforaudioBrucellaspeciesSer

6、ologicalTestingDoxycyclineandrifampinfor6weeksClickiconforaudioBrucellaspeciesTreatmentandPreventionHowlongmustculturesbeheldwhenBrucellaissuspected?Cultureplatesandmediamustbeheldforatleast21daysbecauseBrucellaisaslowgrower.Normalfloraofmouth,respirat

7、ory&GItractsOpportunisticpathogenAssociatedwith:Dental/periodontalandhead/neckinfections/abscessesHumanbitewoundsSepticemiafollowingtoothextractionEndocarditisClickiconforaudioEikenellacorrodensClinicalSignificanceEikenellacorrodensSpecimenCollectionAe

8、robicRequiresheminBAP,CHOCClickiconforaudioTinycoloniesat48hrsColonymorphologyBAP“Pits"theagarPaleyellowpigmentGreeningaroundcolonyBleachodorMAC–nogrowthClickiconforaudioEikenellacorrodensGrowthCharacteristicsEikenellacorrodensIdentific