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1、AerobicGram-PositiveBacilli PartIIDivisionofMedicalTechnologyCarolLarsonMSEd,MT(ASCP)GramstainMorphologyArrangementFormationofsporesCatalasereactionDifferentiationof MajorGPRGeneraSpore-formingGPR,catalasepositiveBacillusspeciesBacillusanthracisBacilluscereusNonspore-f
2、ormingGPR,catalasepositiveListeriamonocytogenesCorynebacteriumspecies(diphtheroids)CorynebacteriumdiphtheriaeCorynebacteriumjekeiumGPRDiscussedinStudentLabNonspore-formingGPR,catalasenegativeGardnerellaspeciesErysipelothrixspeciesLactobacillusspeciesBranchingNocardiofo
3、rmGPRNocardiaspeciesStreptomycesspeciesGPRDiscussedinThisLectureTwotypesShortGPRLong,filamentous GPRNon-sporulatingErysipelothrixrhusiopathiaeGramStainGPR,pleomorphic,Non-sporeforming,CatalasenegativeErysipelothrixrhusiopathiaeColonyMorphologyGrowsonBAPNonhemolyticor a
4、lphahemolyticMicroaerophilicGPR,pleomorphic,Non-sporeforming,CatalasenegativeCatalasenegativeMotilityNonmotileTest-tubebrushgrowthpatterninsemisolidmotilitytubeat48hoursH2SpositiveinKIA/TSI(onlyGPR)Sucrosenon“F”ErysipelothrixrhusiopathiaeIdentificationGPR,pleomorphic,N
5、on-sporeforming,CatalasenegativeZoonoticSwineerysipelas(fataltopigs)Man–skindiseaseErysipelasDirectcontactwithinfectedanimalRarelydisseminatescausingsepticemiawitharthritisorendocarditisErysipelothrixrhusiopathiaeClinicalSignificanceGPR,pleomorphic,Non-sporeforming,Cat
6、alasenegativeWhatarethekeybiochemicalreactionsthatidentifyErysipelothrixrhusiopathiae?Twogramstainmorphologies(GPR),twocolonytypesonSBA,catalasenegative,H2Spositive,test-tubebrushpatterninmotilitytube,sucrosenon“F”TwotypesLongslenderGPRinchainsShortGPCBNon-sporulatingL
7、actobacillusspeciesGramStainGPR,pleomorphic,Non-sporeforming,CatalasenegativeGrowsonBAPMultiplecolonymorphologiesNonhemolyticoralphahemolyticMicroaerophilicLactobacillusspeciesColonyMorphologyGPR,pleomorphic,Non-sporeforming,CatalasenegativeCatalasenegativeSucrose“F”Va
8、ncomycin“R”LactobacillusspeciesIdentificationGPR,pleomorphic,Non-sporeforming,CatalasenegativeLactobacillusspeciesCli
