SterilizationDeviceforLiquidChromatographySolvents液相色谱溶剂灭菌装置

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1、SterilizationDeviceforLiquidChromatographySolventsDesignTeamNickRoulleau,MichaelVoseMichaelRacette,MichaelMcKayAdvisorProfessorMohammadTaslimIntroductionBackgroundProblemStatementPastArtDesignRequirementsDesignConceptsPrototypeDesignComponentAnalysisRecommendationsWhatisLiquidChrom

2、atography?AsubstancecomprisedofcomponentsAandBisdissolvedinasolventandenterstheanalyticalcolumn,whereitisseparatedBasicComponentsofanHPLCSystemFromhttp://www.waters.com/WatersDivisionProblemFromhttp://www.waters.com/WatersDivisionDesignGoalTomitigatetheriskofblockageattheinletfritd

3、uetobacterialcontaminationandextendtheusefullifeoftheUPLCcolumn.ExistingSolutionsIn-linefiltersGuardcolumnsandcartridgesPre-filtrationofsamplesandmobilephaseliquidsProductRequirementsMandatory:MustbeadaptableforuseworldwideMustextendtheusefullifeofcolumnsMustmeetsafetystandards(ISO

4、,ULandCE)Mustoperatefor1yearw/ouserinterventionDesirable:ShouldbeabletofiltertwobottlessimultaneouslyShouldmeetcustomeracceptancecriteriaLow-maintenanceEasytouseCostConstraintsCannotchangethechemicalcompositionOfthesolventOfthesampleCannotcreateriskofcausingpumpcavitationCannothind

5、erbottleaccessibilityCannotnegativelyimpactsystemresolutionInitialDesignConceptsUVProbePump/filter--ExternalenclosurePump/filter--CapenclosurePreliminaryDesign–UVProbeInexpensiveSimpleDesignWhyNotUseUltravioletRadiationasaPrimarySolution?Degradationoforganicsolventmodifiers(LowRisk

6、)Degradationofaqueousadditives(LowRisk)UsersafetyfromUV-Cexposure(MediumRisk)UVcaninactivatebutnotremovebacteriaHowmanybacteriacouldbegeneratedperyear?Logarithmicgrowth:Assumingworstcase100%replicatingShortgenerationtimeNeglectinglagphasesandcelldeathFiltercapacity=107CFU/cm2Filter

7、SizingFilterSizingWithlogarithmicbacterialgrowth,filterareabecomesexceptionallylargeinashortperiodCurrentDesignExternalFilterEnclosurewithUVDual-headbrushlessDCpumpUVlampwithmultiplesterilizationlinesPallAcroPak200filtersFilterSelectionMembranewithmaterialcompatibilitySufficientca

8、pacitytocontain1yearofinac