切向流过滤装置介绍(4)

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1、TFFDevices切向流过滤装置BasicConceptsofTangentialFlowFiltration(TFF)切向流过滤的基本概组件(TFF)MILLIPORE1Outline概要¢TypesofTFFDevices切向流过滤装置的种类Îopenchannel开放型流道Îturbulence-promotedchannels湍流增强型流道Î"enhanced"bysecondaryflow次流道加强型¢Millipore'sdevicesandtheirperformance密理博公司TFF装置及其性能¢RecommendedoperatingconditionsforMilli

2、pore'sTFFdevices密理博公司超滤装置的建议操作条件MILLIPORE2TypesofTFFDevicesTFF装置的类型Openchannel开放流道Flatplates(spacebetweenmembranes)平板(膜中间有空隙)Hollowfibersandtubes中空纤维及管Turbulence-promotedchannel湍流增强型流道Screenbetweenflatsheets/plates每层平板之间有筛网Spiral-wound(screenbetweenflatsheetswoundinaspiralpattern)卷式膜(卷起的膜每层之间有筛网)MI

3、LLIPORE3TFFDeviceSelection--Considerations超滤装置的选择-从全面考虑的角度进行¢Suspendedsolidsinfeed含悬浊的固状物进料Îuseopenchanneltopreventpluggingiftotalsuspendedsolids>2-5%byvolumeÎ如果固状悬浊物总量大于2-5%,使用开放流道型防止堵塞¢Availabilityofchosenmembrane可选用相应的膜usuallyavailableinonly1or2devices通常只有一两种超滤装置可选¢Mechanicalcomplexity,reliabili

4、tyandease-of-use构造复杂,可靠但使用简单¢Simplicityofscale-up线形放大的简单性¢Cost(deviceANDoperatingsystem)成本(设备本身及相应操作系统)MILLIPORE4Microporous(MF)TFFDevices切向流微滤装置(MF)TFFMILLIPORE5TFFDevices,UsesandOperatingConditions切向流(微滤)装置,使用及操作条件Microporous(MF)TFFDevices切向流微滤装置(MF)ÎGlassReinforced(PES)flatplate(Prostak)改良聚醚砜外壳平

5、板膜(Prostak)–Flatplate,openflowchannel平板式,开放流道–(SIP)Steamableinplace(SIP)在线蒸汽灭菌–Minimumvolumes>5L–最小工作体积>5LÎApplications:应用–Cellharvestandclarification细胞收集和澄清–Bacterial,yeast,mammaliancells细菌,酵母,哺乳动物细胞–Lowshearapplications低剪切力应用–Asepticapplications灭菌应用MILLIPORE6Microporous(MF)TFFDevices–Prostak切向流微滤

6、装置-ProstakPermeate透过Retentate回流MILLIPORE7TFFDevices,UsesandOperatingConditions切向流(微滤)装置,使用及操作条件MicroporousTFFDevices切向流微滤装置ÎCassettes(Pellicon)盒式膜包(Pellicon)–Flatsheet,20-meshscreenedchannel平板式,20-MESH筛网–Notsteamable不能蒸汽灭菌–LowerminimumvolumesthanProstak比PROSTAK膜的残留体积更小ÎApplications:应用:Clarification

7、orbacterialcellharvest澄清或细菌,细胞收集-Whenminimumvolumesmustbe100mL-5L处理量范围100mL-5LMaximumsuspendedsolids<5%toavoidplugging最大悬浮固状物的量<5%避免堵塞孔道MILLIPORE8Microporous(MF)TFFDevices–Pellicon切向流微滤装置-PelliconFeed进口Perm