intro to tff 切向流过滤简介

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1、Scientific&TechnicalReportPN33213IntroductiontoTangentialFlowFiltrationforLaboratoryandProcessDevelopmentApplicationsLarrySchwartz,SeniorTechnicalManager,PallLifeSciencesKevinSeeley,Ph.D.,ScientificandLaboratoryServicesIntroductionTangentialflowfiltration(TFF)isarapidandefficientmethod

2、forseparationandpurificationofbiomolecules.Itcanbeappliedtoawiderangeofbiologicalfieldssuchasimmunology,proteinchemistry,molecularbiology,biochemistry,andmicrobiology.TFFcanbeusedtoconcentrateanddesaltsamplesolutionsranginginvolumefrom10mLtothousandsofliters.Itcanbeusedtofractionatelar

3、gefromsmallbiomolecules,harvestcellsuspensions,andclarifyfermentationbrothsandcelllysates.ThisreportdescribesthebasicprinciplesthatgovernTFFandtheuseofTFFcapsulesandcassettesinlaboratoryandprocessdevelopmentapplications.WhyUseTangentialFlowFiltration?1)TFFiseasytosetupanduse–Simplyconn

4、ecttheTFFdevicetoapumpandpressuregauge(s)withtubingandafewfittings,addyoursampletothereservoirandyou’rereadytogo.Anexampleset-upisshowninFigure1.2)TFFisfastandefficient–Itiseasiertosetupandmuchfasterthandialysis.Youcanachievehigherconcentrationsinlesstimethanwithcentrifugaldevicesorst

5、irredcells.3)Performtwostepswithonesystem–Youcanconcentrateanddiafilterasampleonthesamesystem,savingtimeandavoidingproductloss.4)TFFcanbescaled-uporscaled-down.–Materialsofconstructionandcassettepathlengthallowconditionsestablishedduringpilot-scaletrialstobeappliedtoprocess-scaleapplic

6、ations.TFFdevicesareavailablethatcanprocesssamplevolumesassmallas10mLoraslargeasthousandsofliters.5)TFFiseconomical–TFFdevicesandcassettescanbecleanedandreused,ordisposedofaftersingleuse.Asimpleintegritytestcanbeperformedtoconfirmthatmembraneandsealsareintact.WhatCanTangentialFlowFiltr

7、ationDo?1)Concentrateanddesaltproteins(3,6,9)andpeptides2)Concentrateanddesaltnucleicacids[DNA/RNA/oligonucleotides(11)].3)Recoverandpurifyantibodies(4,7)orrecombinantpro-teinsfromcellculturemedia.4)RecoverandpurifyplasmidDNAfromcelllysates(5)orchromosomalDNAfromwholeblood.5)Fraction