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《The application of SIMS to nutrient tracer studiesSIMSin plant physiology 在营养示踪研究中的应用 植物生理学》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、BiolCell(1992)74,127-134127©Elsevier,ParisOriginalarticleTheapplicationofSIMStonutrienttracerstudiesinplantphysiologyDennisLazof,RichardWLinton,RichardJVolk,ThomasWRuftyUSDA-ARS,CRL,POB1168,Oxford,NC27565,USA(Received25November1991;accepted19December1991)Summary-
2、Currentcontroversiesinrootphysiologyrelatingtotheuptakeandtranslocationofmineralnutrientsarepresented.TheopportunitiesforaSIMScontributiontoresolvethesecontroversiesarediscussedforeachofthestableisotopetracersrelevanttoplantnutrition.Itisconcludedthatforallmajorn
3、utrientsexceptphosphorustherearepromisingstableisotopetracers.Atthesametime,therearechallengestoovercomeineachcase,withrespecttobackgroundlevels,peakinterferencesandsometimessensi-tivity.Techniquesoftissuepreparationandhandlingarediscussedinsomedetail,givingatten
4、tiontothespecialrequirementsofplanttissuepreparationandanalysisfordiffusableions.Itissuggestedthatadaptingapreparationandtransfersystem,designedforproductionofbulkfrozen-hydratedcryofracturedspecimens,toasecondaryionmassspectrometerwouldpermiteasy,rapidpreparatio
5、n.Additionally,thispreparationminimizesseveralserioustechnicalproblemsinherenttootherpreparativemethods.Difficul-tiesinquantitationaretreatedbriefly,outliningsomedifficultiesspecifictoplanttissueanalysis.Prospectsforfutureapplicationsinplantmineralnutritionareeva
6、luated,takingintoaccountcurrentinstrumentaldevelopments.cryotechniques/iontransport/plantphysiology/secondaryionmassspectrometry/stableisotopeMassspectrometryinplantnutritionexaminetransportof~SNinshort-termexperiments.Wehave,forexample,beenabletomeasureNO3-influ
7、xMassspectrometryhasnotbeenusedextensivelyinplantintosmallregionsofplantroots(ca1mgDW)fromdi-nutritionresearch.Whenuseditwasprimarilyrelatedtoluteexternalnutrientsolution(100~M[NO3]ext)overtheassimilationofcarbonandnitrogen.Forexample,Eh-time-periodsasbriefas1min
8、(LazofandRufty,unpub-leringerandOsmond's(1989)reviewofstableisotopelishedresults).Whilesensitivitywillalwaysbeordersof(SI)methodsinplantphysiologicalecologyonl
