Chaperone Activity of ERD10 and ERD14, TwoDisordered Stress-Related Plant Proteins1[OA]Erd10和Erd14、2的伴侣活性 无序胁迫相关植物蛋白[1]

《Chaperone Activity of ERD10 and ERD14, TwoDisordered Stress-Related Plant Proteins1[OA]Erd10和Erd14、2的伴侣活性 无序胁迫相关植物蛋白[1]》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、ChaperoneActivityofERD10andERD14,Two1[OA]DisorderedStress-RelatedPlantProteinsDenesKovacs,EvaKalmar,ZsoltTorok,andPeterTompa*InstituteofEnzymology,BiologicalResearchCenter,HungarianAcademyofSciences,Budapest,HungaryH1113(D.K.,P.T.);DepartmentofMedicalChemistry,M

2、olecularBiology,andPathobiochemistry,SemmelweisUniversity,Budapest,HungaryH1088(E.K.);andInstituteofBiochemistry,BiologicalResearchCenter,H6701Szeged,Hungary(Z.T.)ERD10andERD14(forearlyresponsetodehydration)proteinsaremembersofthedehydrinfamilythataccumulateinre

3、sponsetoabioticenvironmentalstresses,suchashighsalinity,drought,andlowtemperature,inArabidopsis(Arabidopsisthaliana).Whereastheseproteinsprotectcellsagainsttheconsequencesofdehydration,theexactmode(s)oftheiractionremainspoorlyunderstood.Here,detailedevidenceispr

4、ovidedthatERD10andERD14belongtothefamilyofintrinsicallydisorderedproteins,anditisshowninvariousassaysthattheyactaschaperonesinvitro.ERD10andERD14areabletopreventtheheat-inducedaggregationand/orinactivationofvarioussubstrates,suchaslysozyme,alcoholdehydrogenase,fi

5、reflyluciferase,andcitratesynthase.ItisalsodemonstratedthatERD10andERD14bindtoacidicphospholipidvesicleswithoutsignificantlyaffectingmembranefluidity.Membranebindingisstronglyinfluencedbyionicstrength.Ourresultsshowthattheseintrinsicallydisorderedproteinshavechapero

6、neactivityofratherwidesubstratespecificityandthattheyinteractwithphospholipidvesiclesthroughelectrostaticforces.Wesuggestthatthesefindingsprovidetherationaleforthemechanismofhowtheseproteinsaverttheadverseeffectsofdehydrationstresses.Lateembryogenesis-abundant(LEA

7、)proteinsareYamaguchi-Shinozaki,1996;Nylanderetal.,2001;Rorat,expressedinlatestagesofseedmaturationand/or2006).RecentresultshaveshownthatsomeoftheDHNsuponwaterstressconditionsinplants(Rorat,2006;alsoaccumulateduringhigh-lightstress(Kimuraetal.,TunnacliffeandWise

8、,2007).Theyconstituteahighly2003).Whereastheseproteinsconfertheplantdesicca-divergentgroupofproteinsthatcanbeclassifiedintotionstresstolerance,theirexactmechanismofact