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1、inalchemHuetal.,MedChem(LosAngeles)2017,7:5dicisetryMMedicinalchemistryDOI:10.4172/2161-0444.1000444ISSN:2161-0444tResearchArticleOpenAccessPharmacokineticInvestigationofMainBioactiveComponentsfromEpimedium-DerivedFlavonoidsinRabbitSerumbyUltra-PerformanceLiquidChromatographyTandemMassSpectrom
2、etryLiufangHu1,2,YuanruZheng1,2,YiZhang1,2,ZifeiQin1,2,3*,YiDai1,2,LinQin4,ZhihongYao1,2*andXinshengYao1,2,31CollegeofPharmacy,JinanUniversity,Guangzhou510632,China2GuangdongProvincialKeyLaboratoryofPharmacodynamicConstituentsofTCMandNewDrugsResearch,CollegeofPharmacy,JinanUniversity,Guangzhou
3、510632,PRChina3IntegratedChineseandWesternMedicinePostdoctoralResearchStation,JinanUniversity,Guangzhou510632,PRChina4MusculoskeletalResearchLaboratory,DepartmentofOrthopedicsandTraumatology,TheChineseUniversityofHongKong,Satin,NTHongKongSAR,PRChinaAbstractBackground:TotalEpimedium-derivedflav
4、onoids(EF)werereportedtobeagroupofmajorbioactiveconstituentspresentingenusEpimediumforthetreatmentofosteonecrosis.ThepresentstudyaimedtoinvestigatethepharmacokineticsofmainbioactivecomponentsofEFinrabbit.Methods:Arapidandsensitivemethodforthesimultaneousdeterminationoficariin,icarisideI,icaris
5、ideIIandicaritininserumwasestablishedandvalidatedbyultra-performanceliquidchromatographycoupledwithquadrupoletime-of-flighttandemmassspectrometry(UPLC-Q-TOF/MS).Serumsampleswerepretreatedwithwater-saturatedethylacetate.Furthermore,sampleseparationwasachievedonanWatersBEHC18column(50mm×2.1mm,1.
6、7μm)bygradientelutionwithacetonitrile-water(bothincluding0.1%formicacid)asmobilephase.Finally,pharmacokineticsofEFinrabbitserumwasinvestigated.Results:Allcalibrationcurvesdisplayedexcellentlinearity(r2>0.99)within1.2~24ng/mL.Theassaywasspecific,preciseandaccurate,asdemonstratedwithintra-runpre
7、cisionsnotmorethan12.6%,inter-runprecisionslessthan9.9%andaccuraciesbetween-4.4%and9.3%.Meanwhile,thematrixeffects,extractionrecoveriesandstabilitieswereallsatisfactory.Moreover,thisvalidatedmethodwassuccessfullyappliedtoanalysethepharm
