常见蛋白质标签

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1、常见蛋白质标签总结2008-12-0822:06Proteintagsarepeptidesequencesgeneticallygraftedontoarecombinantprotein.Oftenthesetagsareremovablebychemicalagentsorbyenzymaticmeans,suchasproteolysisorinteinsplicing.Tagsareattachedtoproteinsforvariouspurposes.一、氨基酸标签（含小肽标签）Astretchofamino

2、acidsisaddedtotheproteinandenablestherecoveryofthelabelledproteinbyitsuniqueaffinity.Usuallyitseasiesttoaddthetagtoeitherendoftheproteintoensureitsaccessibilityandnottodisturbtheproteinfolding.组氨酸标签（Histag）一般为6个组氨酸，用Ni2+（Cu2+）亲和层析纯化FLAGtag：N-DYKDDDDK-C，recoveredwi

3、thspecificantibodyHAtag：anepitopederivedfromtheInfluenzaproteinhaemagglutinin(HA，禽流感病毒血凝素)，e.g.N-YPYDVP-C，recoverywithanHAantibodyMYCtag：anepitopederivedfromthehumanproto-oncoproteinMYC，e.g.N-ILKKATAYIL-C,N-EQKLISEEDL-C，recoverywithanMYCantibodySBPtag：Streptavidin

4、BindingPeptide，链霉亲合素结合肽，38aminoacidtag(MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP)，更多参考在SigmaCBPtag：钙调蛋白结合肽（CBP;26aa）钙调蛋白结合肽与钙调素结合是Ca2+依赖的，这种结合不受标签所处的位置影响（N端和C端均可），在中性pH条件下使用2mMEGTA可以很方便的将目标蛋白洗脱下来。这一系统有如下优点：1特异性很高，因为大肠杆菌没有可以和钙调素结合的蛋白；2与His标签相比可以在强还原性条件下纯化。纤维素结合肽（CBD）：

5、能与纤维素介质特异性的结合，可以在温和的条件下洗脱（乙二醇或低盐条件），pETCBD载体含有纤维素结合肽（CBD）的序列，可方便构建。二、蛋白质标签Ratherthanaddingonlyafewaminoacidsawholeproteinisfusedtotheproteintobepurifiedordetected.Theaffinityoftheattachedproteinenablestherecoveryoftheartificialfusionprotein.Asforthepeptides,thepro

6、teintagisaddedtoeitherendofthetargetprotein.GSTtag：thesmallglutathione-S-transferase(GST;26kDa)，recoverybyaffinitytosubstrateglutathioneboundtoacolumn,e.g.glutathionesepharoseMBPtag：麦芽糖结合蛋白（MBP;40kDa）载体：pMALIMPACT：Intein-MediatedPurificationwithanaffinityChitin-bi

7、ndingTag（几丁质结合肽）硫氧还蛋白：Thioredoxinsareproteinsthatactasantioxidantsbyfacilitatingthereductionofotherproteinsbycysteinethiol-disulfideexchange.ProteinA：a40-60kDaMSCRAMMsurfaceproteinoriginallyfoundinthecellwallofthebacteriaStaphylococcusaureus.Ithasfounduseinbiochem

8、icalresearchbecauseofitsabilitytobindimmunoglobulins.Itbindsproteinsfrommanyofmammalianspecies,mostnotablyIgG’s.ItbindswiththeFcregionofimmunoglobulinst