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ID:40794123
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页数:15页
时间:2019-08-07
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1、PLoSOne.2013;8(9):e74363.Publishedonline2013September3.doi:10.1371/journal.pone.0074363PMCID:PMC3760846PinelliapedatisectaAgglutininTargetsDrugResistantK562/ADRLeukemiaCellsthroughBindingwithSarcolemmalMembraneAssociatedProteinandEnhancingMacrophagePhagocytosisAgglutininTargetsDrugResis
2、tantK562/ADRLeukemiaCellsthroughBindingwithSarcolemmalMembraneAssociatedProteinandEnhancingMacrophagePhagocytosisAgglutininTargetsDrugResistantK562/ADRLeukemiaCellsthroughBindingwithSarcolemmalMembraneAssociatedProteinandEnhancingMacrophagePhagocytosisAgglutininTargetsDrugResistantK562/
3、ADRLeukemiaCellsthroughBindingwithSarcolemmalMembraneAssociatedProteinandEnhancingMacrophagePhagocytosisAgglutininTargetsDrugResistantK562/ADRLeukemiaCellsthroughBindingwithSarcolemmalMembraneAssociatedProteinandEnhancingMacrophagePhagocytosisCollegeofLifeSciences,ZhejiangSci-TechUniver
4、sity,Hangzhou,Zhejiang,ChinaAgglutininTargetsDrugResistantK562/ADRLeukemiaCellsthroughBindingwithSarcolemmalMembraneAssociatedProteinandEnhancingMacrophagePhagocytosisKanChen,XinyanYang,LiqinWu,MeilanYu,XiaoyanLi,NaLi,ShuanghuiWang,andGongchuLi*SophiaNKaragiannis,EditorAuthorinformation
5、►Articlenotes►CopyrightandLicenseinformation►Goto:AbstractPinelliapedatisectaagglutinin(PPA)haspreviouslybeenusedinlabelingfractionsofmyeloidleukemiacellsinourlaboratory.WereportherethatabacterialexpressedrecombinantPPAdomainbtaggedwithsolublecoxsackieandadenovirusreceptor(sCAR-PPAb)pre
6、ferentiallyrecognizeddrugresistantcancercellsK562/ADRandH460/5Fu,ascomparedtotheirparentalcelllines.PretreatmentofK562/ADRcellswithsCAR-PPAbsignificantlyenhancedphagocytosisofK562/ADRbymacrophagesinvivo.Meanwhile,inaK562/ADRxenograftmodel,intratumoralinjectionofsCAR-PPAbinducedmacrophag
7、einfiltrationandphagocytosis.Furthermore,immunoprecipitation,massspectrometryandWesternblotidentifiedthemembranetargetofPPAonK562/ADRassarcolemmalmembraneassociatedprotein(SLMAP).AnantibodyagainstSLMAPsignificantlypromotedthephagocytosisofK562/ADRbymacrophagesinvitro.Thesefindi
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