2015-PCaP2 regulates nuclear positioning in growing Arabidopsis thaliana root hairs by modulating filamentous actin organization.

2015-PCaP2 regulates nuclear positioning in growing Arabidopsis thaliana root hairs by modulating filamentous actin organization.

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时间:2019-08-06

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1、PlantCellRep(2015)34:1317–1330DOI10.1007/s00299-015-1789-6ORIGINALPAPERPCaP2regulatesnuclearpositioningingrowingArabidopsisthalianaroothairsbymodulatingfilamentousactinorganization11111YanZhang•ErfangKang•MingYuan•YingFu•LeiZhuReceived:15February2015/Revi

2、sed:23March2015/Accepted:31March2015/Publishedonline:1May2015ÓSpringer-VerlagBerlinHeidelberg2015AbstractKeywordsNucleusRoothairTipgrowthActinKeymessagePCaP2playsakeyroleinmaintainingfilamentActin-bindingproteinArabidopsisthalianathenucleusatarelativ

3、elyfixeddistancefromtheapexduringroothairgrowthbymodulatingactinfilaments.AbstractDuringroothairgrowth,thenucleuslocalizesataIntroductionrelativelyfixeddistancefromtheapex.InArabidopsisthaliana,thepositionofthenucleusismainlydependentonRoothairsaretubularpr

4、otrusionsonrootepidermalcells.theconfigurationofmicrofilaments(filamentousactin).TheformationofroothairsexpandstherootsurfaceareaHowever,themechanismsunderlyingtheregulationofactinandfacilitatestheuptakeofwaterandnutrientsfromthedynamicsandorganizationfornu

5、clearpositioningarelargelysurroundingenvironment(Dolanetal.1994;Ketelaarandunknown.Inthepresentstudy,wedemonstratedthatplasmaEmons2001,2009;Libaultetal.2010).Roothairsexhibit2?membrane-associatedCabindingprotein2(PCaP2)in-typicaltipgrowth,whichisregulate

6、dbyavarietyoffac-fluencesthepositionofthenucleusduringroothairgrowth.tors,includingcytoskeletonelements,vesiculartransport,AbnormalexpressionofPCaP2inpcap2andPCaP2over-andcellwallsynthesisprocesses(CarolandDolan2002;expressionplantsledtothedisorganization

7、ofactinfilaments,Dietetal.2006;Ovecˇkaetal.2005;Peietal.2012).Fineratherthanmicrotubules,intheapexandsub-apicalregionsfilamentousactininthesub-apicalregionofgrowingrootofroothairs,whichresultedinaberrantroothairgrowthhairslikelyplaysanimportantroleinthereg

8、ulationofrootpatternsandmisplacednuclei.AnalysesusingaPCaP2mu-hairtipgrowthbydeliveringnewmembraneandcellwalltantproteinrevealedthatactin-severingactivityisessentialmaterialtothegrowthsite(Milleretal.1999;Fanetal.forthefun

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