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Water–Polysaccharide Interactions in the Primary Cell Wall of Arabidopsis thaliana from Polarization Transfer Solid-State NMR

Water–Polysaccharide Interactions in the Primary Cell Wall of Arabidopsis thaliana from Polarization Transfer Solid-State NMR

ID：40729603

大小：2.67 MB

页数：11页

时间：2019-08-06

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1、Articlepubs.acs.org/JACSWater−PolysaccharideInteractionsinthePrimaryCellWallofArabidopsisthalianafromPolarizationTransferSolid-StateNMR†,§†,§‡‡,†PaulB.White,TuoWang,YongBumPark,DanielJ.Cosgrove,andMeiHong*†DepartmentofChemistryandAmesLaboratory,IowaStateUniversity,Ames,I

2、owa50011,UnitedStates‡DepartmentofBiology,PennsylvaniaStateUniversity,UniversityPark,Pennsylvania16802,UnitedStates*SSupportingInformationABSTRACT:Polysaccharide-richplantcellwallsarehydratedunderfunctionalconditions,butthemolecularinteractionsbetweenwaterandpolysacchari

3、desinthewallhavenotbeeninvestigated.Inthiswork,weemploypolarizationtransfersolid-stateNMRtechniquestostudythehydrationofprimary-wallpolysaccharidesofthemodelplant,Arabidopsisthaliana.Bytransferringwater1Hpolarizationtopolysaccharidesthroughdistance-andmobility-dependent1

4、H−1Hdipolarcouplingsanddetectingitthroughpolysaccharide13Csignals,weobtaininformationaboutwaterproximitytocellulose,hemicellulose,andpectinsaswellaswatermobility.Bothintactandpartiallyextractedcellwallsamplesarestudied.Ourresultsshowthatwater−pectinpolarizationtransferis

5、muchfasterthanwater−cellulosepolarizationtransferinallsamples,buttheextentofextractionhasaprofoundimpactonthewater−polysaccharidespindiffusion.Removalofcalciumionsandtheconsequentextractionofhomogalacturonan(HG)significantlysloweddownspindiffusion,whilefurtherextractionofma

6、trixpolysaccharidesrestoredthespindiffusionrate.Thesetrendsareobservedincellwallswithsimilarwatercontent,thustheyreflectinherentdifferencesinthemobilityandspatialdistributionofwater.Combinedwithquantitativeanalysisofthepolysaccharidecontents,ourresultsindicatethatcalciumion

7、sandHGgelationincreasetheamountofboundwater,whichfacilitatesspindiffusion,whilecalciumremovaldisruptsthegelandgivesrisetohighlydynamicwater,whichslowsdownspindiffusion.Therecoveryofspindiffusionratesaftermoreextensiveextractionisattributedtoincreasedwater-exposedsurfacearea

8、softhepolysaccharides.Water−pectinspindiffusionprecedeswater−cellulosespindiffusion,lendingsupporttothesi

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