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1、Rheumatology2013;52:16091618RHEUMATOLOGYdoi:10.1093/rheumatology/ket214AdvanceAccesspublication17June2013OriginalarticleExpressionofWntpathwaymediatorsinmetaplasictissueinanimalmodelandclinicalsamplesoftendinopathy12,32,34,5PaulinePoYeeLui,YukWaLee,YinMeiWong,X
2、iaolingZhang,4,56KerongDaiandChristerGustavRolfDownloadedfromAbstractObjective.Tissuemetaplasiaisobservedinbothossifiedfailedhealinganimalmodelandclinicalsamplesoftendinopathy.TheWntsignallingpathwayplaysavitalroleinpathologicalcalcification.Wehypothe-sizedthat
3、theWntsignallingpathwaymightcontributetotissuemetaplasiaandfailedhealingintendino-pathy.Thisstudyaimedtoexaminethespatial-temporalexpressionofWntpathwaymediatorsinanhttp://rheumatology.oxfordjournals.org/ossifiedfailedtendonhealinganimalmodelandclinicalsampleso
4、ftendinopathy.TheeffectofWnt3aontheosteogenicdifferentiationoftendon-derivedstemcells(TDSCs)wasalsoexamined.Methods.Ossifiedfailedtendonhealingwasinducedbytheinjectionofcollagenaseintothepatellartendonofrats.Atvarioustimesthetendonswereharvestedforimmunohistoch
5、emicalstainingofWnt3a,b-catenin,Lrp5andTcf1.Patellartendonsampleswereobtainedfrom13patientswithpatellartendino-pathy(11unossifiedand2ossified)and10controls.ImmunohistochemicalstainingofWnt3a,b-catenin,Lrp5andTcf1wassimilarlyperformed.RatpatellarTDSCsweretreated
6、withWnt3a.TheosteogenicdifferentiationofTDSCswasexaminedbyALPactivity,alizarinredSstainingandmRNAexpressionofosteogenicmarkers.atZhejiangUniversityonNovember30,2013Results.TherewasincreasedexpressionofWnt3a,b-catenin,Lrp5andTcf1inthehealingfibroblast-likecells,
7、chondrocyte-likecellsandossifieddepositsintheanimalmodelandinsomeclinicalsamplesoftendinopathy.Wnt3aincreasedALPactivity,calciumnoduleformationandexpressionofosteogenicmarkersinTDSCs.Conclusion.ActivationoftheWntsignallingpathwayanditseffectonTDSCsmightcontribu
8、tetotissuemetaplasiaandfailedhealinginsomecasesoftendinopathy.BASICSCIENCEKeywords:Wntsignalling,tendinopathy,ossification,tendonstem/progenitorcells,tendon-derivedstemcells
