【2】New advances in molecular recognition based on biomolecular

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1、AnalBioanalChem(2013)405:5679–5685DOI10.1007/s00216-013-6983-1TRENDSNewadvancesinmolecularrecognitionbasedonbiomolecularscaffoldsMeipingZhao&TongboWu&XianjinXiao&YangLiu&XinSuReceived:12March2013/Revised:9April2013/Accepted:11April2013/Publishedonline:9May20

2、13#Springer-VerlagBerlinHeidelberg2013AbstractInthisreview,wesummarizerecentadvancesinpurposes[1–3].Thecommonlyusedmolecularrecognitionthedevelopmentofmolecularrecognitioncomponentselementsaregenerallyoffourdifferenttypes:proteins/basedonthebiomolecularscaff

3、oldsofproteinsandnucleicpeptides,nucleicacids/oligonucleotides,smallorganicmole-acidsforspecificrecognitionofmiscellaneoustargets.Incules,andsyntheticpolymers.Agreatdealofscientificatten-additiontothewidelyadoptedrecombinantantibodyfrag-tionhasbeenpaidtothes

4、eimportantcomponents,andthements,designedankyrinrepeatproteinsandmodularpep-technologyofproducingandutilizingthebioaffinity-basedtiderepeatsoftranscription-activator-likeeffectorsforbase-recognitionmoleculesisevolvingveryfast.Herewepresentaspecificrecognitio

5、nofDNAsequencearealsobrieflyintro-summaryofrecentadvancesintheconstructionandimprove-duced.Forthenucleicacidbasedmolecularrecognitionmentofmolecularrecognitionelementsbasedonthefirsttwosystems,aptamers,includingslowoff-ratemodifiedclassesofbiomolecules.Progr

6、essregardingsmallorganicaptamers,DNAzymes,andsyntheticDNA-likeoligomersmoleculeorsynthetic-polymer-basedmolecularrecognitionforversatilebiorecognitionaredescribed.Finally,wedis-componentscanbefoundinotherreviews[4–7].cusstheremainingchallengesandfutureresear

7、chdirectionsProteinsorpeptidesaremadefromapoolof20naturallyinthefield.occurringaminoacidbuildingblocks,resultinginlibrarieswithmorechemicaldiversitythannucleicacids,whichareKeywordsRecombinantantibody.Engineeredprotein.madefromfivenaturallyoccurringnucleotid

8、ebuildingAptamer.DNAzyme.SyntheticDNA-likeoligomer.blocks.Nowadays,antibodiesarestillplayingapredomi-Invitroselectionnantroleinthediagnosticandtherapeuticfields.Antibody-basedbioassaysarewidelyu