高通量测序技术介绍及其应用

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1、目录一、高通量测序技术背景知识....................................................................................11、什么是测序......................................................................................................1Sanger法测序....................................................................

2、............................1高通量测序....................................................................................................12、常见的测序平台介绍......................................................................................2IlluminaSolexa测序技术....................................

3、.........................................2Roche454测序技术......................................................................................2ABISOLiD测序技术...................................................................................33、高通量测序技术的应用......................................

4、............................................1二、各类型测序技术介绍及其使用目的*..................................................................21、基因组重测序..................................................................................................22、Denovo测序........................................

5、...........................................................33、外显子测序（WholeExonSequencing）......................................................34、转录组测序（RNA-seq）...............................................................................35、小RNA测序（SmallRNASequencing）....................

6、.................................46、ChIP-seq测序..................................................................................................47、CHIRP-Seq测序..............................................................................................48、CLIP-seq测序......................

7、...........................................................................59、宏基因组测序（MetagenomeSequencing）.................................................5三、不同类型高通量测序技术在基础医学领域中的应用*......................................61、Exome-seq在医学研究中的应用......................................