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1、中国医学科学院学报ACTAACADEMIAEMEDICINAESINICAE·论著·流式荧光杂交法检测高危型人乳头瘤病毒的临床验证试验123123廖光东,张小燕,高玉华,刘彬,刘霞,李联昆,1211陈凤,卞美璐,陈汶,乔友林1中国医学科学院北京协和医学院肿瘤研究所肿瘤流行病学研究室,北京1000212中日友好医院妇产科,北京1000293辽宁省肿瘤医院妇科,沈阳110042通信作者:乔友林电话/传真:010267713648,电子邮件:qiaoy@public1bta1net1cn摘要:目的评估流式荧光杂交法检测高危型人乳头瘤病毒(HPV)的临床应用价
2、值。方法随机选择1061名年龄在21～65岁间的妇女,采用流式荧光杂交法和第二代杂交捕获实验(hc2)检测其子宫颈脱落细胞,以病理诊断作为金标准对检测结果进行分析和评价,并对二者的检测结果作一致性检验。结果流式荧光杂交法检测的灵敏度和特异度分别为80131%和85183%。阳、阴性预测值分别为4415%和9619%。与hc2检测一致性检验结果显示Kappa=0158。结论流式荧光杂交法检测高危型HPV的特异度与hc2相似,灵敏度低于hc2,但两者检测的一致性较好。关键词:子宫颈癌;人乳头瘤病毒;检测分型;流式荧光杂交法中图分类号:R37319;R737
3、133文献标识码:A文章编号:10002503X(2007)0520603205ClinicalEvaluationofthexMAPTechnologyinDetectionofHigh2riskHumanPapillomavirus12312LIAOGuang2dong,ZHANGXiao2yan,GAOYu2hua,LIUBin,LIUXia,31211LILian2kun,CHENFeng,BIANMei2lu,CHENWen,QIAOYou2lin1DepartmentofCancerEpidemiology,CancerInstitute,
4、CAMSandPUMC,Beijing100021,China2DepartmentofGynecologyandObstetrics,China2JapanFriendshipHospital,Beijing100029,China3DepartmentofGynecology,LiaoningProvincialTumorHospital,Shenyang110042,ChinaCorrespondingauthor:QIAOYou2linTel/Fax:010267713648,E2mail:qiaoy@public1bta1net1cnABST
5、RACT:ObjectiveToevaluatetheclinicalapplicationvalueofflexiblemulti2analyteprofiling(xMAP)technologyindetectinghigh2riskhumanpapillomavirus(HR2HPV)1MethodsTotally1061women,aged21265years,wererandomlyenrolledintothestudy1CervicalexfoliatedcellswereusedinxMAPtechnologyandhybridcapt
6、ureⅡ(hc2)1Pathologicaldiagnosiswasusedasgoldenstandard1Consistencyofthesetwomethodswasassessed1ResultsThesensitivityandspecificityofxMAPtechnologywere80131%and85183%,respectively1Thepositiveandnegativepredictivevalueswere4415%and9619%,respective2ly1TheKappavalueforconsistencybet
7、weenxMAPtechnologyandhc2was01581ConclusionsThespecifici2tyofxMAPtechnologyissimilartohc2test,butthesensitivityisinferiortohc21However,thesetwomethodsshowgoodconsistencyinthedetectionofHR2HPV1Keywords:cervicalcancer;humanpapillomavirus;detectionandgenotyping;flexiblemulti2analyte
8、profilingtechnologyActaAcadMedSin,2007,29(5):60
