A protoplast to plant system for the chrysanthemum Dendranthema zawadskii x D. grandiflora

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1、PlantCellReports(1993)12:278-280PlantCeUReports©Springer-Verlag1993AprotoplasttoplantsystemforthechrysanthemumDendranthemazawadskiixD.grandifloraGraemeC.Lindsay1andSusanE.Ledger1,2iMAFTechnology,MinistryofAgricultureandFisheries,HorticulturalResearchCentre,PrivateBag,Levin,

2、NewZealandzPresentaddress."Dept.ofCellularandMolecularBiology,UniversityofAuckland,PrivateBag,Auckland,NewZealandReceivedSeptember24,1990/RevisedversionreceivedNovember28,1992-CommunicatedbyEConstabelSummary.Plantletswereregeneratedfrombeforetechniquessuchassomatichybridiza

3、tionprotoplastsofinvitroshootculturesandleaf-ortransformationthroughdirectDNAuptakederiveddenovoshootsofthechrysanthemumcanbeusedtointroducevaluabletraitsintoDendranthemazawadskiixD.grandiflora.cultivatedspecies.IsolatedprotoplastsreformedcellwallsandthenTherecalcitrantnatu

4、reofchrysanthemumbegantodividewithin24hoursofcultureinprotoplastshasmeantthatithasnotbeenstreakyplateagaroselensessurroundedbyliquidpossibletousethesetechniquestodevelopV-KMmedium.Twentyonedaysafterisolation,superiorplantmaterial.Therearefewreports1mmdiametercallusclumpswer

5、etransferredtoofchrysanthemumprotoplastsproceedingfromshootregenerationmedium.Afterafurther33celldivisiontocellclustersandoccasionallydaysleavesbecamevisible.Elongatedshootscallus(SchumandPreil1981;Okamuraetal.wererootedonhalfstrengthhormone-freeMS1984;AmagasaandKameya1989;

6、Kumarmedium.1985).RecentlySauvadetetal.(1990)reportedshootregenerationfromacommercialcultivarKeyWords:Chrysanthemum-DendranthemaofDendranthemagrandiflora.zawadskiixD.grandiflora-Protoplast-InthispaperwedescribeanefficientandRegenerationreproducibleprocedureforplantletregene

7、rationfromprotoplastsofaKoreangenotypeofAbbreviations:BAP=6-benzylaminopurine;Dendranthema.Agrobacterium-mediatedIAA=3-indoleaceticacid;MS=MurashigeandtransformationofthismaterialhaspreviouslySkoog(1962);NAA=l-naphthylaceticacid;beenreportedfromthislaboratorybyLedgeretPfr=P

8、hotonfluencerate;V-KM=BindingandaL(1991).TheyextensivelytestedtheresponseNehls(197