资源描述：
《101-UV-VIS Absorption Spectra》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、101Adopted:12May1981OECDGUIDELINEFORTESTINGOFCHEMICALS"UV-VISAbsorptionSpectra"(SpectrophotometricMethod)1.INTRODUCTORYINFORMATION•Guidanceinformation–Molecularformula–Structuralformula•StandarddocumentsThespectrophotometricmethodisbasedonnationalstandardsandconsensusmethodswhichareappliedtomeasure
2、theabsorptionspectra.2.METHODA.INTRODUCTION,PURPOSE,SCOPE,RELEVANCE,APPLICATIONANDLIMITSOFTESTTheprimaryenvironmentalpurposeindeterminingtheultraviolet-visible(UV-VIS)absorptionspectrumofachemicalcompoundistohavesomeindicationofthewavelengthsatwhichthecompoundsmaybesusceptibletophotochemicaldegrada
3、tion.Sincephotochemicaldegradationislikelytooccurinboththeatmosphereandtheaquaticenvironment,spectraappropriatetothesemediawillbeinformativeconcerningtheneedforfurtherpersistencetesting.Degradationwilldependuponthetotalenergyabsorbedinspecificwavelengthregions.Suchenergyabsorptionischaracterisedbyb
4、othmolarabsorptioncoefficient(molarextinctioncoefficient)andbandwidth.However,theabsenceofmeasurableabsorptiondoesnotprecludethepossibilityofphotodegradation.•DefinitionsandunitsTheUV-VISabsorptionspectrumofasolutionisafunctionoftheconcentration,ci,expressedinmol/l,ofallabsorbingspeciespresent;thep
5、athlength,d,ofthespectrophotometercell,expressedincm;andthemolarabsorption(extinction)coefficient,i,ofeachspecies.Theabsorbance(opticaldensity)Aofthesolutionisthengivenby:A=dåeiciiUsersofthisTestGuidelineshouldconsultthePreface,inparticularparagraphs3,4,7and8.101page2"UV-VISAbsorptionSpectra"Forare
6、solvableabsorbancepeak,thebandwidthisthewavelengthrange,expressedin-9nm=10m,ofthepeakathalftheabsorbancemaximum.•ReferencesubstancesThereferencesubstancesneednotbeemployedinallcaseswheninvestigatinganewsubstance.Theyareprovidedprimarilysothatcalibrationofthemethodmaybeperformedfromtimetotimeandtoof
7、ferthechancetocomparetheresultswhenanothermethodisapplied.Referencecompounds,appropriateforthecalibrationofthesystemare:(1)potassiumdichromate(in0.005mol/L,H2SO4solution)fromJ.A.A.Ketelaar(2)log3.563.633.16
